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of virally infected cells by CD8+ T cells requires differentiation between self and nonself peptide-class I major histocompatibility complexes (pMHC). Recognition of foreign pMHC by host T cells is a major factor in the rejection of transplanted organs from the same species (allotransplant) or different species (xenotransplant). AHIII12.2 is a murine T cell clone that recognizes the xenogeneic (human) class I MHC HLA-A2.1 molecule (A2) and the syngeneic murine class I MHC H-2 D(b) molecule (D(b)). Recognition of both A2 and D(b) are peptide-dependent, and the sequences of the peptides recognized have been determined. Alterations in the antigenic peptides bound to A2 cause large changes in AHIII12.2 T cell responsiveness. Crystal structures of three representative peptides (agonist, null, and antagonist) bound to A2 partially explain the changes in AHIII12.2 responsiveness. Using class I pMHC octamers, a strong correlation is seen between T cell activity and the affinity of pMHC complexes for the T cell receptor. However, contrary to previous studies, we see similar half-lives for the pMHC multimers bound to the AHIII12.2 cell surface.","bibjson":{"author":[{"initials":"J","lastname":"Buslepp","name":"Buslepp J"},{"initials":"R","lastname":"Zhao","name":"Zhao R"},{"initials":"D","lastname":"Donnini","name":"Donnini D"},{"initials":"D","lastname":"Loftus","name":"Loftus D"},{"initials":"M","lastname":"Saad","name":"Saad M"},{"initials":"E","lastname":"Appella","name":"Appella E"},{"initials":"EJ","lastname":"Collins","name":"Collins EJ"}],"identifier":[{"id":"10.1074/jbc.M109231200","type":"doi"},{"id":"11584024","type":"pubmed"}],"issue":["50"],"journal":{"iso_abbreviation":"J. Biol. 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