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identify and consider some characteristics of a peptide antagonist for the Ag-specific receptor on 2C cells (the 2C TCR). The peptide, GNYSFYAL (called GNY), binds to H-2K(b), and a very high-resolution crystal structure of the GNY-K(b) complex at 1.35 A is described. Although the GNY peptide does not bind to L(d), the potency of GNY-K(b) as an antagonist is evident from its ability to specifically inhibit 2C TCR-mediated reactions to an allogenic agonist complex (QLSPFPFDL-L(d)), as well as to a syngeneic agonist complex (SIYRYYGL-K(b)). The crystal structure and the activities of alanine-substituted peptide variants point to the properties of the peptide P4 side chain and the conformation of the Tyr-P6 side chain as the structural determinants of GNYSFYAL antagonist activity.","bibjson":{"author":[{"initials":"MG","lastname":"Rudolph","name":"Rudolph MG"},{"initials":"LQ","lastname":"Shen","name":"Shen LQ"},{"initials":"SA","lastname":"Lamontagne","name":"Lamontagne SA"},{"initials":"JG","lastname":"Luz","name":"Luz JG"},{"initials":"JR","lastname":"Delaney","name":"Delaney JR"},{"initials":"Q","lastname":"Ge","name":"Ge Q"},{"initials":"BK","lastname":"Cho","name":"Cho BK"},{"initials":"D","lastname":"Palliser","name":"Palliser D"},{"initials":"CA","lastname":"McKinley","name":"McKinley CA"},{"initials":"J","lastname":"Chen","name":"Chen J"},{"initials":"IA","lastname":"Wilson","name":"Wilson IA"},{"initials":"HN","lastname":"Eisen","name":"Eisen HN"}],"identifier":[{"id":"10.4049/jimmunol.172.5.2994","type":"doi"},{"id":"14978103","type":"pubmed"}],"issue":["5"],"journal":{"iso_abbreviation":"J. Immunol.","name":""},"pages":["2994-3002"],"title":"A peptide that antagonizes TCR-mediated reactions with both syngeneic and allogeneic agonists: functional and structural aspects.","type":"article","url":"http://www.jimmunol.org/lookup/doi/10.4049/jimmunol.172.5.2994","volume":["172"],"year":[2004]},"in_pmc":"N","in_pmce":"N","open_access":"N"},"resolution":"1.35","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/1lk2"},"rcsb":{"url":"https://www.rcsb.org/structure/1lk2"}},"species":{"common_name":"Mouse","match_type":"histo:assign_species","scientific_name":"Mus musculus","slug":"mus_musculus"},"tcr":null,"title":"H2-Kb binding \"GNYSFYAL\" at 1.35Å resolution","unique_chain_count":3}}