{"display":true,"structure":{"allele":{"alpha":{"identifier":"ipd-imgt:HLA31926","locus":"hla-b","match_type":"histo:exact","name":"HLA-B*35:08","slug":"hla_b_35_08","species_stem":"hla"},"beta":{}},"assemblies":{"1":{"assembly_name":"2fz3_1","chains":["A","B","C"],"downloaded_at":"2022-11-09T14:40:40.194226"}},"assembly_count":null,"assigned_chains":{"beta2m":{"chains":["B"],"match_type":"histo:assign_chains","score":1.0,"sequence":"IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM"},"class_i_alpha":{"chains":["A"],"match_type":"histo:assign_chains","score":0.91,"sequence":"GSHSMRYFYTAMSRPGRGEPRFIAVGYVDDTQFVRFDSDAASPRTEPRAPWIEQEGPEYWDRNTQIFKTNTQTYRESLRNLRGYYNQSEAGSHIIQRMYGCDLGPDGRLLRGHDQSAYDGKDYIALNEDLSSWTAADTAAQITQRKWEAARVAEQRRAYLEGLCVEWLRRYLENGKETLQRADPPKTHVTHHPVSDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEP"},"peptide":{"chains":["C"],"match_type":"histo:classify_peptide","sequence":"HPVGEADYFEY"}},"chronology":{"deposition_date":"2006-02-09","deposition_year":2006,"release_date":"2006-12-26","release_year":2006,"revision_date":"2019-11-06","revision_year":2019},"class":"class_i","classical":true,"complex_type":"class_i_with_peptide","components":["class_i_alpha","beta2m","peptide"],"experimental_method":"X-ray diffraction","facets":{},"ligands":[],"locus":"hla-b","manually_edited":{},"missing_residues":[],"page_title":"2FZ3 | HLA-B*35:08 binding \"HPVGEADYFEY\"","pdb_code":"2fz3","pdb_title":"The role of T cell receptor alpha genes in directing human MHC restriction","peptide":{"C":{"actual_length":11,"actual_sequence":"HPVGEADYFEY","chain_id":"C","features":["correct_sequence_and_length"],"full_length":11,"full_sequence":"HPVGEADYFEY"}},"peptide_length":11,"peptide_length_name":"undecamer","peptide_neighbours":{"1":{"neighbours":[{"position":167,"residue":"TRP"},{"position":63,"residue":"ASN"},{"position":5,"residue":"MET"},{"position":171,"residue":"TYR"},{"position":33,"residue":"PHE"},{"position":59,"residue":"TYR"},{"position":7,"residue":"TYR"},{"position":159,"residue":"TYR"},{"position":62,"residue":"ARG"},{"position":66,"residue":"ILE"}],"position":1,"residue":"HIS"},"10":{"neighbours":[{"position":77,"residue":"SER"},{"position":76,"residue":"GLU"},{"position":146,"residue":"LYS"},{"position":147,"residue":"TRP"},{"position":73,"residue":"THR"},{"position":80,"residue":"ASN"}],"position":10,"residue":"GLU"},"11":{"neighbours":[{"position":146,"residue":"LYS"},{"position":147,"residue":"TRP"},{"position":116,"residue":"SER"},{"position":124,"residue":"ILE"},{"position":96,"residue":"GLN"},{"position":84,"residue":"TYR"},{"position":74,"residue":"TYR"},{"position":97,"residue":"ARG"},{"position":95,"residue":"ILE"},{"position":80,"residue":"ASN"},{"position":142,"residue":"ILE"},{"position":77,"residue":"SER"},{"position":143,"residue":"THR"},{"position":123,"residue":"TYR"},{"position":81,"residue":"LEU"}],"position":11,"residue":"TYR"},"2":{"neighbours":[{"position":9,"residue":"TYR"},{"position":66,"residue":"ILE"},{"position":99,"residue":"TYR"},{"position":63,"residue":"ASN"},{"position":67,"residue":"PHE"},{"position":7,"residue":"TYR"},{"position":159,"residue":"TYR"}],"position":2,"residue":"PRO"},"3":{"neighbours":[{"position":99,"residue":"TYR"},{"position":156,"residue":"ARG"},{"position":159,"residue":"TYR"},{"position":9,"residue":"TYR"},{"position":66,"residue":"ILE"},{"position":97,"residue":"ARG"},{"position":70,"residue":"ASN"},{"position":155,"residue":"GLN"}],"position":3,"residue":"VAL"},"4":{"neighbours":[{"position":62,"residue":"ARG"},{"position":66,"residue":"ILE"},{"position":156,"residue":"ARG"}],"position":4,"residue":"GLY"},"5":{"neighbours":[{"position":97,"residue":"ARG"},{"position":70,"residue":"ASN"},{"position":9,"residue":"TYR"},{"position":156,"residue":"ARG"}],"position":5,"residue":"GLU"},"6":{"neighbours":[{"position":155,"residue":"GLN"}],"position":6,"residue":"ALA"},"8":{"neighbours":[{"position":73,"residue":"THR"}],"position":8,"residue":"TYR"},"9":{"neighbours":[{"position":152,"residue":"VAL"},{"position":73,"residue":"THR"},{"position":77,"residue":"SER"},{"position":156,"residue":"ARG"},{"position":147,"residue":"TRP"},{"position":74,"residue":"TYR"},{"position":97,"residue":"ARG"}],"position":9,"residue":"PHE"}},"peptide_sequence":"HPVGEADYFEY","pockets":{"a":{"a159":{"number":159,"one_letter":"Y","three_letter":"TYR"},"a163":{"number":163,"one_letter":"L","three_letter":"LEU"},"a167":{"number":167,"one_letter":"W","three_letter":"TRP"},"a171":{"number":171,"one_letter":"Y","three_letter":"TYR"},"a5":{"number":5,"one_letter":"M","three_letter":"MET"},"a59":{"number":59,"one_letter":"Y","three_letter":"TYR"},"a63":{"number":63,"one_letter":"N","three_letter":"ASN"},"a66":{"number":66,"one_letter":"I","three_letter":"ILE"},"a7":{"number":7,"one_letter":"Y","three_letter":"TYR"}},"b":{"a24":{"number":24,"one_letter":"A","three_letter":"ALA"},"a34":{"number":34,"one_letter":"V","three_letter":"VAL"},"a45":{"number":45,"one_letter":"T","three_letter":"THR"},"a63":{"number":63,"one_letter":"N","three_letter":"ASN"},"a66":{"number":66,"one_letter":"I","three_letter":"ILE"},"a67":{"number":67,"one_letter":"F","three_letter":"PHE"},"a7":{"number":7,"one_letter":"Y","three_letter":"TYR"},"a70":{"number":70,"one_letter":"N","three_letter":"ASN"},"a9":{"number":9,"one_letter":"Y","three_letter":"TYR"},"a99":{"number":99,"one_letter":"Y","three_letter":"TYR"}},"c":{"a70":{"number":70,"one_letter":"N","three_letter":"ASN"},"a73":{"number":73,"one_letter":"T","three_letter":"THR"},"a74":{"number":74,"one_letter":"Y","three_letter":"TYR"},"a9":{"number":9,"one_letter":"Y","three_letter":"TYR"},"a97":{"number":97,"one_letter":"R","three_letter":"ARG"}},"d":{"a114":{"number":114,"one_letter":"D","three_letter":"ASP"},"a155":{"number":155,"one_letter":"Q","three_letter":"GLN"},"a156":{"number":156,"one_letter":"R","three_letter":"ARG"},"a159":{"number":159,"one_letter":"Y","three_letter":"TYR"},"a160":{"number":160,"one_letter":"L","three_letter":"LEU"},"a99":{"number":99,"one_letter":"Y","three_letter":"TYR"}},"e":{"a114":{"number":114,"one_letter":"D","three_letter":"ASP"},"a147":{"number":147,"one_letter":"W","three_letter":"TRP"},"a152":{"number":152,"one_letter":"V","three_letter":"VAL"},"a156":{"number":156,"one_letter":"R","three_letter":"ARG"},"a97":{"number":97,"one_letter":"R","three_letter":"ARG"}},"f":{"a116":{"number":116,"one_letter":"S","three_letter":"SER"},"a123":{"number":123,"one_letter":"Y","three_letter":"TYR"},"a143":{"number":143,"one_letter":"T","three_letter":"THR"},"a146":{"number":146,"one_letter":"K","three_letter":"LYS"},"a147":{"number":147,"one_letter":"W","three_letter":"TRP"},"a77":{"number":77,"one_letter":"S","three_letter":"SER"},"a80":{"number":80,"one_letter":"N","three_letter":"ASN"},"a81":{"number":81,"one_letter":"L","three_letter":"LEU"},"a84":{"number":84,"one_letter":"Y","three_letter":"TYR"},"a95":{"number":95,"one_letter":"I","three_letter":"ILE"}}},"publication":{"abstract":"The underlying generic properties of alphabeta TCRs that control MHC restriction remain largely unresolved. To investigate MHC restriction, we have examined the CTL response to a viral epitope that binds promiscuously to two human leukocyte Ags (HLAs) that differ by a single amino acid at position 156. Individuals expressing either HLA-B*3501 (156Leucine) or HLA-B*3508 (156Arginine) showed a potent CTL response to the 407HPVGEADYFEY417 epitope from EBV. Interestingly, the response was characterized by highly restricted TCR beta-chain usage in both HLA-B*3501+ and HLA-B*3508+ individuals; however, this conserved TRBV9+ beta-chain was associated with distinct TCR alpha-chains depending upon the HLA-B*35 allele expressed by the virus-exposed host. Functional assays confirmed that TCR alpha-chain usage determined the HLA restriction of the CTLs. Structural studies revealed significant differences in the mobility of the peptide when bound to HLA-B*3501 or HLA-B*3508. In HLA-B*3501, the bulged section of the peptide was disordered, whereas in HLA-B*3508 the bulged epitope adopted an ordered conformation. Collectively, these data demonstrate not only that mobile MHC-bound peptides can be highly immunogenic but can also stimulate an extremely biased TCR repertoire. In addition, TCR alpha-chain usage is shown to play a critical role in controlling MHC restriction between closely related allomorphs.","bibjson":{"author":[{"initials":"JJ","lastname":"Miles","name":"Miles JJ"},{"initials":"NA","lastname":"Borg","name":"Borg NA"},{"initials":"RM","lastname":"Brennan","name":"Brennan RM"},{"initials":"FE","lastname":"Tynan","name":"Tynan FE"},{"initials":"L","lastname":"Kjer-Nielsen","name":"Kjer-Nielsen L"},{"initials":"SL","lastname":"Silins","name":"Silins SL"},{"initials":"MJ","lastname":"Bell","name":"Bell MJ"},{"initials":"JM","lastname":"Burrows","name":"Burrows JM"},{"initials":"J","lastname":"McCluskey","name":"McCluskey J"},{"initials":"J","lastname":"Rossjohn","name":"Rossjohn J"},{"initials":"SR","lastname":"Burrows","name":"Burrows SR"}],"identifier":[{"id":"10.4049/jimmunol.177.10.6804","type":"doi"},{"id":"17082594","type":"pubmed"}],"issue":["10"],"journal":{"iso_abbreviation":"J. Immunol.","name":""},"pages":["6804-14"],"title":"TCR alpha genes direct MHC restriction in the potent human T cell response to a class I-bound viral epitope.","type":"article","url":"http://www.jimmunol.org/lookup/doi/10.4049/jimmunol.177.10.6804","volume":["177"],"year":[2006]},"in_pmc":"N","in_pmce":"N","open_access":"N"},"resolution":"1.90","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/2fz3"},"rcsb":{"url":"https://www.rcsb.org/structure/2fz3"}},"species":{"common_name":"Human","match_type":"histo:assign_species","scientific_name":"Homo sapiens","slug":"homo_sapiens"},"tcr":null,"title":"HLA-B*35:08 binding \"HPVGEADYFEY\" at 1.90Å resolution","unique_chain_count":3}}