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Overall, the structure is extremely similar to that of HLA-A2 (refs 1, 2; and M.A.S. et al., manuscript in preparation), although the 11 amino-acid substitutions at polymorphic residues in the antigen-binding cleft alter the detailed shape and electrostatic charge of that site. A prominent negatively charged pocket within the cleft extends underneath the alpha-helix of the alpha 1-domain, providing a potential subsite for recognizing a positively charged side chain or peptide N terminus. Uninterpreted electron density, presumably representing an unknown 'antigen(s)', which seems to be different from that seen in the HLA-A2 structure, occupies the cleft and extends into the negatively charged pocket in HLA-Aw68. The structures of HLA-Aw68 and HLA-A2 demonstrate how polymorphism creates and alters subsites (pockets) positioned to bind peptide side chains, thereby suggesting the structural basis for allelic specificity in foreign antigen binding.","bibjson":{"author":[{"initials":"TP","lastname":"Garrett","name":"Garrett TP"},{"initials":"MA","lastname":"Saper","name":"Saper MA"},{"initials":"PJ","lastname":"Bjorkman","name":"Bjorkman PJ"},{"initials":"JL","lastname":"Strominger","name":"Strominger JL"},{"initials":"DC","lastname":"Wiley","name":"Wiley DC"}],"identifier":[{"id":"10.1038/342692a0","type":"doi"},{"id":"2594067","type":"pubmed"}],"issue":["6250"],"journal":{"iso_abbreviation":"Nature","name":""},"pages":["692-6"],"title":"Specificity pockets for the side chains of peptide antigens in HLA-Aw68.","type":"article","url":"http://www.nature.com/articles/342692a0","volume":["342"],"year":[1989]},"in_pmc":"N","in_pmce":"N","open_access":"N"},"resolution":"2.60","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/2hla"},"rcsb":{"url":"https://www.rcsb.org/structure/2hla"}},"species":{"common_name":"Human","match_type":"histo:assign_species","scientific_name":"Homo sapiens","slug":"homo_sapiens"},"tcr":null,"title":"HLA-A*68:01 possibly without \"peptide\" at 2.60Å resolution","unique_chain_count":2}}