{"display":true,"structure":{"allele":{"alpha":{"identifier":"ipd-imgt:HLA35266","locus":"hla-a","match_type":"histo:exact","name":"HLA-A*02:01","slug":"hla_a_02_01","species_stem":"hla"},"beta":{}},"assemblies":{"1":{"assembly_name":"3tvm_1","chains":["A","B","C","D","I","J"],"downloaded_at":"2022-11-09T14:45:55.853662"}},"assembly_count":null,"assigned_chains":{"beta2m":{"chains":["B","F"],"match_type":"histo:assign_chains","score":1.0,"sequence":"IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVEMSDMSFSKDWSFYILAHTEFTPTETDTYACRVKHASMAEPKTVYWDRDM"},"cd1d":{"chains":["A","E"],"match_type":"histo:assign_chains","score":0.97,"sequence":"SEAQQKNYTFRCLQMSSFANRSWSRTDSVVWLGDLQTHRWSNDSATISFTKPWSQGKLSNQQWEKLQHMFQVYRVSFTRDIQELVKMMSPKEDYPIEIQLSAGCEMYPGNASESFLHVAFQGKYVVRFWGTSWQTVPGAPSWLDLPIKVLNADQGTSATVQMLLNDTCPLFVRGLLEAGKSDLEKQEKPVAWLSSVPSSAHGHRQLVCHVSGFYPKPVWVMWMRGDQEQQGTHRGDFLPNADETWYLQATLDVEAGEEAGLACRVKHSSLGGQDIILYWHHHHHH"},"tcr_alpha":{"chains":["C"],"match_type":"stcrdab","sequence":"MKTQVEQSPQSLVVRQGENCVLQCNYSVTPDNHLRWFKQDTGKGLVSLTVLVDQKDKTSNGRYSATLDKDAKHSTLHITATLLDDTATYICVVGDRGSALGRLHFGAGTQLIVIPDIQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKCVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESS","subgroup":"TRAV11"},"tcr_beta":{"chains":["D"],"match_type":"stcrdab","sequence":"MEAAVTQSPRNKVAVTGGKVTLSCNQTNNHNNMYWYRQDTGHGLRLIHYSYGAGSTEKGDIPDGYKASRPSQENFSLILELATPSQTSVYFCASGDEGYTQYFGPGTRLLVLEDLRNVTPPKVSLFEPSKAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVCTDPQPLKEQPALNDSRYSLSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRA","subgroup":"TRBV13"}},"chronology":{"deposition_date":"2011-09-20","deposition_year":2011,"release_date":"2012-02-01","release_year":2012,"revision_date":"2020-07-29","revision_year":2020},"class":"class_i","classical":false,"complex_type":"cd1d_with_nkt_alpha_beta_tcr","components":["cd1d","beta2m","tcr_alpha","tcr_beta"],"experimental_method":"X-ray diffraction","facets":{},"ligands":[],"locus":"hla-a","manually_edited":{},"missing_residues":[],"page_title":"3TVM | Non-classical MHC Class I molecule CD1d with Natural Killer Alpha/Beta T cell receptor","pdb_code":"3tvm","pdb_title":"Structure of the mouse CD1d-smc124-inkt TCR complex","peptide":{"actual_sequence":null,"epitope_info":{},"features":[],"full_sequence":null,"gap_info":{},"gapped_sequence":null,"length":{"numeric":null,"text":null},"unnatural_amino_acids":[]},"publication":{"abstract":"Natural killer T (NKT) cells recognize glycolipids presented by CD1d. The first antigen described, \u03b1-galactosyl ceramide (\u03b1GalCer), is a potential anticancer agent whose activity depends upon IFN-\u03b3 secretion. We report two analogs of \u03b1GalCer based on a naturally occurring glycosphingolipid, plakoside A. These compounds induce enhanced IFN-\u03b3 that correlates with detergent-resistant binding to CD1d and an increased stability of the lipid-CD1d complexes on antigen-presenting cells. Structural analysis on one of the analogs indicates that it is more deeply bound inside the CD1d groove, suggesting tighter lipid-CD1d interactions. To our knowledge, this is the first example in which structural information provides an explanation for the increased lipid-CD1d stability, likely responsible for the Th1 bias. We provide insights into the mechanism of IFN-\u03b3-inducing compounds, and because our compounds activate human NKT cells, they could have therapeutic utility.","bibjson":{"author":[{"initials":"AJ","lastname":"Tyznik","name":"Tyznik AJ"},{"initials":"E","lastname":"Farber","name":"Farber E"},{"initials":"E","lastname":"Girardi","name":"Girardi E"},{"initials":"A","lastname":"Birkholz","name":"Birkholz A"},{"initials":"Y","lastname":"Li","name":"Li Y"},{"initials":"S","lastname":"Chitale","name":"Chitale S"},{"initials":"R","lastname":"So","name":"So R"},{"initials":"P","lastname":"Arora","name":"Arora P"},{"initials":"A","lastname":"Khurana","name":"Khurana A"},{"initials":"J","lastname":"Wang","name":"Wang J"},{"initials":"SA","lastname":"Porcelli","name":"Porcelli SA"},{"initials":"DM","lastname":"Zajonc","name":"Zajonc DM"},{"initials":"M","lastname":"Kronenberg","name":"Kronenberg M"},{"initials":"AR","lastname":"Howell","name":"Howell AR"}],"identifier":[{"id":"10.1016/j.chembiol.2011.10.015","type":"doi"},{"id":"22195564","type":"pubmed"}],"issue":["12"],"journal":{"iso_abbreviation":"Chem. Biol.","name":""},"pages":["1620-30"],"title":"Glycolipids that elicit IFN-\ufffd\ufffd-biased responses from natural killer T cells.","type":"article","url":"https://www.sciencedirect.com/science/article/abs/pii/S1074552111004005","volume":["18"],"year":[2011]},"in_pmc":"Y","in_pmce":"Y","open_access":"N"},"resolution":"2.80","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/3tvm"},"rcsb":{"url":"https://www.rcsb.org/structure/3tvm"},"stcrdab":{"url":"http://opig.stats.ox.ac.uk/webapps/stcrdab/StrViewer?pdb=3tvm"}},"species":{"common_name":"Mouse","match_type":"histo:assign_species","scientific_name":"Mus musculus","slug":"mus_musculus"},"tcr":{"alpha":{"chains":["C"],"subgroup":"TRAV11"},"beta":{"chains":["D"],"subgroup":"TRBV13"},"pdb_code":"3tvm"},"title":"Non-classical MHC Class I molecule CD1d with Natural Killer Alpha/Beta T cell receptor at 2.80Å resolution","unique_chain_count":4}}