{"display":true,"structure":{"allele":{"alpha":{"identifier":"ipd-imgt:HLA34811","locus":"hla-b","match_type":"histo:exact","name":"HLA-B*27:05","slug":"hla_b_27_05","species_stem":"hla"},"beta":{}},"assemblies":{"1":{"assembly_name":"4l4v_1","chains":["A","B","G","H"],"downloaded_at":"2022-11-09T14:27:43.139333"}},"assembly_count":null,"assigned_chains":{"beta2m":{"chains":["B","F"],"match_type":"histo:assign_chains","score":1.0,"sequence":"IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM"},"mr1":{"chains":["A","C"],"match_type":"histo:assign_chains","score":1.0,"sequence":"MRTHSLRYFRLGVSDPIHGVPEFISVGYVDSHPITTYDSVTRQKEPRAPWMAENLAPDHWERYTQLLRGWQQMFKVELKRLQRHYNHSGSHTYQRMIGCELLEDGSTTGFLQYAYDGQDFLIFNKDTLSWLAVDNVAHTIKQAWEANQHELLYQKNWLEEECIAWLKRFLEYGKDTLQRTEPPLVRVNRKETFPGVTALFCKAHGFYPPEIYMTWMKNGEEIVQEIDYGDILPSGDGTYQAWASIELDPQSSNLYSCHVEHSGVHMVLQVP"},"tcr_alpha":{"chains":["D"],"match_type":"stcrdab","sequence":"GQNIDQPTEMTATEGAIVQINCTYQTSGFNGLFWYQQHAGEAPTFLSYNVLDGLEEKGRFSSFLSRSKGYSYLLLKELQMKDSASYLCAVKDSNYQLIWGAGTKLIIKPDIQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKCVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESS","subgroup":"TRAV1"},"tcr_beta":{"chains":["E"],"match_type":"stcrdab","sequence":"NAGVTQTPKFQVLKTGQSMTLQCAQDMNHNSMYWYRQDPGMGLRLIYYSASEGTTDKGEVPNGYNVSRLNKREFSLRLESAAPSQTSVYFCASSVWTGEGSGELFFGEGSRLTVLEDLKNVFPPEVAVFEPSEAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVCTDPQPLKEQPALNDSRYALSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRAD","subgroup":"TRBV6"}},"chronology":{"deposition_date":"2013-06-09","deposition_year":2013,"release_date":"2013-07-17","release_year":2013,"revision_date":"2013-09-11","revision_year":2013},"class":"class_i","classical":false,"complex_type":"mr1_with_alpha_beta_tcr","components":["mr1","beta2m","tcr_alpha","tcr_beta"],"experimental_method":"X-ray diffraction","facets":{},"ligands":[],"locus":"hla-b","manually_edited":{},"missing_residues":[],"page_title":"4L4V | Non-classical MHC Class I molecule MR1 with Alpha/Beta T cell receptor","pdb_code":"4l4v","pdb_title":"Structure of human MAIT TCR in complex with human MR1-rl-6-me-7-oh","peptide":{"actual_sequence":null,"epitope_info":{},"features":[],"full_sequence":null,"gap_info":{},"gapped_sequence":null,"length":{"numeric":null,"text":null},"unnatural_amino_acids":[]},"publication":{"abstract":"The mucosal-associated invariant T-cell antigen receptor (MAIT TCR) recognizes MR1 presenting vitamin B metabolites. Here we describe the structures of a human MAIT TCR in complex with human MR1 presenting a non-stimulatory ligand derived from folic acid and an agonist ligand derived from a riboflavin metabolite. For both vitamin B antigens, the MAIT TCR docks in a conserved manner above MR1, thus acting as an innate-like pattern recognition receptor. The invariant MAIT TCR \u03b1-chain usage is attributable to MR1-mediated interactions that prise open the MR1 cleft to allow contact with the vitamin B metabolite. Although the non-stimulatory antigen does not contact the MAIT TCR, the stimulatory antigen does. This results in a higher affinity of the MAIT TCR for a stimulatory antigen in comparison with a non-stimulatory antigen. We formally demonstrate a structural basis for MAIT TCR recognition of vitamin B metabolites, while illuminating how TCRs recognize microbial metabolic signatures.","bibjson":{"author":[{"initials":"O","lastname":"Patel","name":"Patel O"},{"initials":"L","lastname":"Kjer-Nielsen","name":"Kjer-Nielsen L"},{"initials":"J","lastname":"Le Nours","name":"Le Nours J"},{"initials":"SB","lastname":"Eckle","name":"Eckle SB"},{"initials":"R","lastname":"Birkinshaw","name":"Birkinshaw R"},{"initials":"T","lastname":"Beddoe","name":"Beddoe T"},{"initials":"AJ","lastname":"Corbett","name":"Corbett AJ"},{"initials":"L","lastname":"Liu","name":"Liu L"},{"initials":"JJ","lastname":"Miles","name":"Miles JJ"},{"initials":"B","lastname":"Meehan","name":"Meehan B"},{"initials":"R","lastname":"Reantragoon","name":"Reantragoon R"},{"initials":"ML","lastname":"Sandoval-Romero","name":"Sandoval-Romero ML"},{"initials":"LC","lastname":"Sullivan","name":"Sullivan LC"},{"initials":"AG","lastname":"Brooks","name":"Brooks AG"},{"initials":"Z","lastname":"Chen","name":"Chen Z"},{"initials":"DP","lastname":"Fairlie","name":"Fairlie DP"},{"initials":"J","lastname":"McCluskey","name":"McCluskey J"},{"initials":"J","lastname":"Rossjohn","name":"Rossjohn J"}],"identifier":[{"id":"10.1038/ncomms3142","type":"doi"},{"id":"23846752","type":"pubmed"}],"issue":[null],"journal":{"iso_abbreviation":"Nat Commun","name":""},"pages":["2142"],"title":"Recognition of vitamin B metabolites by mucosal-associated invariant T cells.","type":"article","url":"http://www.nature.com/articles/ncomms3142","volume":["4"],"year":[2013]},"in_pmc":"N","in_pmce":"N","open_access":"N"},"resolution":"1.90","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/4l4v"},"rcsb":{"url":"https://www.rcsb.org/structure/4l4v"},"stcrdab":{"url":"http://opig.stats.ox.ac.uk/webapps/stcrdab/StrViewer?pdb=4l4v"}},"species":{"common_name":"Human","match_type":"histo:assign_species","scientific_name":"Homo sapiens","slug":"homo_sapiens"},"tcr":{"alpha":{"chains":["D"],"subgroup":"TRAV1"},"beta":{"chains":["E"],"subgroup":"TRBV6"},"pdb_code":"4l4v"},"title":"Non-classical MHC Class I molecule MR1 with Alpha/Beta T cell receptor at 1.90Å resolution","unique_chain_count":4}}