{"display":true,"structure":{"allele":{"alpha":{"identifier":"uniprot:P01897","locus":"h2-l","match_type":"histo:fuzzy","name":"H2-Ld","slug":"h2_ld","species_stem":"H2"},"beta":{}},"assemblies":{"1":{"assembly_name":"4lcw_1","chains":["A","B","G","H"],"downloaded_at":"2022-11-09T14:42:24.362948"}},"assembly_count":null,"assigned_chains":{"beta2m":{"chains":["B","F"],"match_type":"histo:assign_chains","score":1.0,"sequence":"IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM"},"mr1":{"chains":["A","C"],"match_type":"histo:assign_chains","score":1.0,"sequence":"MRTHSLRYFRLGVSDPIHGVPEFISVGYVDSHPITTYDSVTRQAEPRAPWMAENLAPDHWERYTQLLRGWQQMFKVELKRLQRHYNHSGSHTYQRMIGCELLEDGSTTGFLQYAYDGQDFLIFNKDTLSWLAVDNVAHTIKQAWEANQHELLYQKNWLEEECIAWLKRFLEYGKDTLQRTEPPLVRVNRKETFPGVTALFCKAHGFYPPEIYMTWMKNGEEIVQEIDYGDILPSGDGTYQAWASIELDPQSSNLYSCHVEHSGVHMVLQVP"},"tcr_alpha":{"chains":["D"],"match_type":"stcrdab","sequence":"GQNIDQPTEMTATEGAIVQINCTYQTSGFNGLFWYQQHAGEAPTFLSYNVLDGLEEKGRFSSFLSRSKGYSYLLLKELQMKDSASYLCAVKDSNYQLIWGAGTKLIIKPDIQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKCVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESS","subgroup":"TRAV1"},"tcr_beta":{"chains":["E"],"match_type":"stcrdab","sequence":"NAGVTQTPKFQVLKTGQSMTLQCAQDMNHNSMYWYRQDPGMGLRLIYYSASEGTTDKGEVPNGYNVSRLNKREFSLRLESAAPSQTSVYFCASSVWTGEGSGELFFGEGSRLTVLEDLKNVFPPEVAVFEPSEAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVCTDPQPLKEQPALNDSRYALSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRAD","subgroup":"TRBV6"}},"chronology":{"deposition_date":"2013-06-24","deposition_year":2013,"release_date":"2013-10-02","release_year":2013,"revision_date":"2013-11-20","revision_year":2013},"class":"class_i","classical":false,"complex_type":"mr1_with_alpha_beta_tcr","components":["mr1","beta2m","tcr_alpha","tcr_beta"],"experimental_method":"X-ray diffraction","facets":{},"ligands":[],"locus":"h2-l","manually_edited":{},"missing_residues":[],"page_title":"4LCW | Non-classical MHC Class I molecule MR1 with Alpha/Beta T cell receptor","pdb_code":"4lcw","pdb_title":"The structure of human MAIT TCR in complex with MR1-k43a-rl-6-me-7oh","peptide":{"actual_sequence":null,"epitope_info":{},"features":[],"full_sequence":null,"gap_info":{},"gapped_sequence":null,"length":{"numeric":null,"text":null},"unnatural_amino_acids":[]},"publication":{"abstract":"Mucosal-associated invariant T cells (MAIT cells) express a semi-invariant T cell receptor (TCR) \u03b1-chain, TRAV1-2-TRAJ33, and are activated by vitamin B metabolites bound by the major histocompatibility complex (MHC)-related class I-like molecule, MR1. Understanding MAIT cell biology has been restrained by the lack of reagents to specifically identify and characterize these cells. Furthermore, the use of surrogate markers may misrepresent the MAIT cell population. We show that modified human MR1 tetramers loaded with the potent MAIT cell ligand, reduced 6-hydroxymethyl-8-D-ribityllumazine (rRL-6-CH\u2082OH), specifically detect all human MAIT cells. Tetramer(+) MAIT subsets were predominantly CD8(+) or CD4(-)CD8(-), although a small subset of CD4(+) MAIT cells was also detected. Notably, most human CD8(+) MAIT cells were CD8\u03b1(+)CD8\u03b2(-/lo), implying predominant expression of CD8\u03b1\u03b1 homodimers. Tetramer-sorted MAIT cells displayed a T(H)1 cytokine phenotype upon antigen-specific activation. Similarly, mouse MR1-rRL-6-CH\u2082OH tetramers detected CD4(+), CD4(-)CD8(-) and CD8(+) MAIT cells in V\u03b119 transgenic mice. Both human and mouse MAIT cells expressed a broad TCR-\u03b2 repertoire, and although the majority of human MAIT cells expressed TRAV1-2-TRAJ33, some expressed TRAJ12 or TRAJ20 genes in conjunction with TRAV1-2. Accordingly, MR1 tetramers allow precise phenotypic characterization of human and mouse MAIT cells and revealed unanticipated TCR heterogeneity in this population.","bibjson":{"author":[{"initials":"R","lastname":"Reantragoon","name":"Reantragoon R"},{"initials":"AJ","lastname":"Corbett","name":"Corbett AJ"},{"initials":"IG","lastname":"Sakala","name":"Sakala IG"},{"initials":"NA","lastname":"Gherardin","name":"Gherardin NA"},{"initials":"JB","lastname":"Furness","name":"Furness JB"},{"initials":"Z","lastname":"Chen","name":"Chen Z"},{"initials":"SB","lastname":"Eckle","name":"Eckle SB"},{"initials":"AP","lastname":"Uldrich","name":"Uldrich AP"},{"initials":"RW","lastname":"Birkinshaw","name":"Birkinshaw RW"},{"initials":"O","lastname":"Patel","name":"Patel O"},{"initials":"L","lastname":"Kostenko","name":"Kostenko L"},{"initials":"B","lastname":"Meehan","name":"Meehan B"},{"initials":"K","lastname":"Kedzierska","name":"Kedzierska K"},{"initials":"L","lastname":"Liu","name":"Liu L"},{"initials":"DP","lastname":"Fairlie","name":"Fairlie DP"},{"initials":"TH","lastname":"Hansen","name":"Hansen TH"},{"initials":"DI","lastname":"Godfrey","name":"Godfrey DI"},{"initials":"J","lastname":"Rossjohn","name":"Rossjohn J"},{"initials":"J","lastname":"McCluskey","name":"McCluskey J"},{"initials":"L","lastname":"Kjer-Nielsen","name":"Kjer-Nielsen L"}],"identifier":[{"id":"10.1084/jem.20130958","type":"doi"},{"id":"24101382","type":"pubmed"}],"issue":["11"],"journal":{"iso_abbreviation":"J. Exp. Med.","name":""},"pages":["2305-20"],"title":"Antigen-loaded MR1 tetramers define T cell receptor heterogeneity in mucosal-associated invariant T cells.","type":"article","url":"https://rupress.org/jem/article/210/11/2305/41566/Antigenloaded-MR1-tetramers-define-T-cell-receptor","volume":["210"],"year":[2013]},"in_pmc":"Y","in_pmce":"Y","open_access":"Y"},"resolution":"2.40","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/4lcw"},"rcsb":{"url":"https://www.rcsb.org/structure/4lcw"},"stcrdab":{"url":"http://opig.stats.ox.ac.uk/webapps/stcrdab/StrViewer?pdb=4lcw"}},"species":{"common_name":"Human","match_type":"histo:assign_species","scientific_name":"Homo sapiens","slug":"homo_sapiens"},"tcr":{"alpha":{"chains":["D"],"subgroup":"TRAV1"},"beta":{"chains":["E"],"subgroup":"TRBV6"},"pdb_code":"4lcw"},"title":"Non-classical MHC Class I molecule MR1 with Alpha/Beta T cell receptor at 2.40Å resolution","unique_chain_count":4}}