{"display":true,"structure":{"allele":{"alpha":{"identifier":"ipd-mhc:BoLA03187","locus":"bola-2","match_type":"histo:fuzzy","name":"BoLA-2*018:01","slug":"bola_2_018_01","species_stem":"BoLA"},"beta":{}},"assemblies":{"1":{"assembly_name":"4mng_1","chains":["B","A","E"],"downloaded_at":"2022-11-09T14:33:03.950729"}},"assembly_count":null,"assigned_chains":{"beta2m":{"chains":["B","D"],"match_type":"histo:assign_chains","score":1.0,"sequence":"IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVEMSDMSFSKDWSFYILAHTEFTPTETDTYACRVKHASMAEPKTVYWDRDM"},"cd1d":{"chains":["A","C"],"match_type":"histo:assign_chains","score":0.73,"sequence":"ADPVPQRLFPLRCLQISSFANSSWTRTDGLAWLGELQTHSWSNDSDTVRSLKPWSQGTFSDQQWETLQHIFRVYRSSFTRDVKEFAKMLRLSYPLELQVSAGCEVHPGNASNNFFHVAFQGKDILSFQGTSWEPTQEAPLWVNLAIQVLNQDKWTRETVQWLLNGTCPQFVSGLLESGKSELKKQEKPVAWLSSVPSSAHGHRQLVCHVSGFYPKPVWVMWMRGDQEQQGTHRGDFLPNADETWYLQATLDVEAGEEAGLACRVKHSSLGGQDIILYWSGR"},"tcr_delta":{"chains":["E"],"match_type":"stcrdab","sequence":"AQKVTQAQSSVSMPVRKAVTLNCLYETSWWSYYIFWYKQLPSKEMIFLIRQGSDEQNAKSGRYSVNFKKAAKSVALTISALQLEDSAKYFCALGEPSYWGFPRTTRVIFGKGTRVTVEPGGGGSGGGGSGGGGSGGGGSSSNLEGRTKSVIRQTGSSAEITCDLAEGSTGYIHWYLHQEGKAPQRLLYYDSYTSSVVLESGISPGKYDTYGSTRKNLRMILRNLIENDSGVYYCATWDEKYYKKLFGSGTKLIITDAASGAD","subgroup":"TRDV1"}},"chronology":{"deposition_date":"2013-09-10","deposition_year":2013,"release_date":"2013-12-18","release_year":2013,"revision_date":"2020-07-29","revision_year":2020},"class":"class_i","classical":false,"complex_type":"cd1d_with_single_chain_trc","components":["cd1d","beta2m","tcr_delta"],"experimental_method":"X-ray diffraction","facets":{},"ligands":[],"locus":"bola-2","manually_edited":{},"missing_residues":[],"page_title":"4MNG | Non-classical MHC Class I molecule CD1d with single chain gamma TCR construct","pdb_code":"4mng","pdb_title":"Structure of the dp10.7 TCR with CD1d-sulfatide","peptide":{"actual_sequence":null,"epitope_info":{},"features":[],"full_sequence":null,"gap_info":{},"gapped_sequence":null,"length":{"numeric":null,"text":null},"unnatural_amino_acids":[]},"publication":{"abstract":"The nature of the antigens recognized by \u03b3\u03b4 T cells and their potential recognition of major histocompatibility complex (MHC)-like molecules has remained unclear. Members of the CD1 family of lipid-presenting molecules are suggested ligands for V\u03b41 TCR-expressing \u03b3\u03b4 T cells, the major \u03b3\u03b4 lymphocyte population in epithelial tissues. We crystallized a V\u03b41 TCR in complex with CD1d and the self-lipid sulfatide, revealing the unusual recognition of CD1d by germline V\u03b41 residues spanning all complementarity-determining region (CDR) loops, as well as sulfatide recognition separately encoded by nongermline CDR3\u03b4 residues. Binding and functional analysis showed that CD1d presenting self-lipids, including sulfatide, was widely recognized by gut V\u03b41+ \u03b3\u03b4 T cells. These findings provide structural demonstration of MHC-like recognition of a self-lipid by \u03b3\u03b4 T cells and reveal the prevalence of lipid recognition by innate-like T cell populations.","bibjson":{"author":[{"initials":"AM","lastname":"Luoma","name":"Luoma AM"},{"initials":"CD","lastname":"Castro","name":"Castro CD"},{"initials":"T","lastname":"Mayassi","name":"Mayassi T"},{"initials":"LA","lastname":"Bembinster","name":"Bembinster LA"},{"initials":"L","lastname":"Bai","name":"Bai L"},{"initials":"D","lastname":"Picard","name":"Picard D"},{"initials":"B","lastname":"Anderson","name":"Anderson B"},{"initials":"L","lastname":"Scharf","name":"Scharf L"},{"initials":"JE","lastname":"Kung","name":"Kung JE"},{"initials":"LV","lastname":"Sibener","name":"Sibener LV"},{"initials":"PB","lastname":"Savage","name":"Savage PB"},{"initials":"B","lastname":"Jabri","name":"Jabri B"},{"initials":"A","lastname":"Bendelac","name":"Bendelac A"},{"initials":"EJ","lastname":"Adams","name":"Adams EJ"}],"identifier":[{"id":"10.1016/j.immuni.2013.11.001","type":"doi"},{"id":"24239091","type":"pubmed"}],"issue":[null],"journal":{"iso_abbreviation":"Immunity","name":""},"pages":[null],"title":"Crystal Structure of V\ufffd\ufffd1\ufffd\ufffdT Cell Receptor in Complex with CD1d-Sulfatide Shows MHC-like Recognition of a Self-Lipid by Human \ufffd\ufffd\ufffd\ufffd T Cells.","type":"article","url":"https://www.sciencedirect.com/science/article/abs/pii/S1074761313004780","volume":[null],"year":[2013]},"in_pmc":"N","in_pmce":"Y","open_access":"N"},"resolution":"3.01","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/4mng"},"rcsb":{"url":"https://www.rcsb.org/structure/4mng"},"stcrdab":{"url":"http://opig.stats.ox.ac.uk/webapps/stcrdab/StrViewer?pdb=4mng"}},"species":{"common_name":"Human","match_type":"histo:assign_species","scientific_name":"Homo sapiens","slug":"homo_sapiens"},"tcr":{"delta":{"chains":["E"],"subgroup":"TRDV1"},"gamma":{"chains":["e"],"subgroup":"TRGV4"},"pdb_code":"4mng"},"title":"Non-classical MHC Class I molecule CD1d with single chain gamma TCR construct at 3.01Å resolution","unique_chain_count":3}}