{"display":true,"structure":{"allele":{"alpha":{"identifier":"ipd-imgt:HLA34051","locus":"hla-b","match_type":"histo:exact","name":"HLA-B*57:01","slug":"hla_b_57_01","species_stem":"hla"},"beta":{}},"assemblies":{"1":{"assembly_name":"5b39_1","chains":["A","B","C","G"],"downloaded_at":"2022-11-09T14:31:44.611479"}},"assembly_count":null,"assigned_chains":{"beta2m":{"chains":["B"],"match_type":"histo:assign_chains","score":1.0,"sequence":"IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM"},"class_i_alpha":{"chains":["A"],"match_type":"histo:assign_chains","score":0.92,"sequence":"GSHSMRYFYTAMSRPGRGEPRFIAVGYVDDTQFVRFDSDAASPRMAPRAPWIEQEGPEYWDGETRNMKASAQTYRENLRIALRYYNQSEAGSHIIQVMYGCDVGPDGRLLRGHDQSAYDGKDYIALNEDLSSWTAADTAAQITQRKWEAARVAEQLRAYLEGLCVEWLRRYLENGKETLQRADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEP"},"kir3":{"chains":["G"],"match_type":"histo:assign_chains","score":0.95,"sequence":"HVGGQDKPFLSAWPSAVVPRGGHVTLRCHYRHRFNNFMLYKEDRIHVPIFHGRLFQESFNMSPVTTAHAGNYTCRGSHPHSPTGWSAPSNPVVIMVTGNHRKPSLLAHPGPLVKSGERVILQCWSDIMFEHFFLHKEGISKDPSRLVGQIHDGVSKANFSIGPMMLALAGTYRCYGSVTHTPYQLSAPSDPLDIVVTGPYEKPSLSAQPGPKVQAGESVTLSCSSRSSYDMYHLSREGGAHERRLPAVRKVNRTFQADFPLGPATHGGTYRCFGSFRHSPYEWSDPSDPLLVSVTGNPS"},"peptide":{"chains":["C"],"match_type":"histo:classify_peptide","sequence":"LSSPVTKSF"}},"chronology":{"deposition_date":"2016-02-12","deposition_year":2016,"release_date":"2016-03-30","release_year":2016,"revision_date":"2020-07-29","revision_year":2020},"class":"class_i","classical":true,"complex_type":"class_i_with_peptide_and_kir3","components":["class_i_alpha","beta2m","peptide","kir3"],"experimental_method":"X-ray diffraction","facets":{},"ligands":[],"locus":"hla-b","manually_edited":{},"missing_residues":[],"page_title":"5B39 | HLA-B*57:01 binding \"LSSPVTKSF\" with KIR-3 NK receptor","pdb_code":"5b39","pdb_title":"Kir3dl1*015 in complex with hla-b*57:01","peptide":{"C":{"actual_length":9,"actual_sequence":"LSSPVTKSF","chain_id":"C","features":["correct_sequence_and_length"],"full_length":9,"full_sequence":"LSSPVTKSF"}},"peptide_length":9,"peptide_length_name":"nonamer","peptide_neighbours":{"1":{"neighbours":[{"position":33,"residue":"PHE"},{"position":171,"residue":"TYR"},{"position":63,"residue":"GLU"},{"position":163,"residue":"LEU"},{"position":159,"residue":"TYR"},{"position":59,"residue":"TYR"},{"position":7,"residue":"TYR"},{"position":5,"residue":"MET"},{"position":167,"residue":"TRP"}],"position":1,"residue":"LEU"},"2":{"neighbours":[{"position":67,"residue":"MET"},{"position":9,"residue":"TYR"},{"position":45,"residue":"MET"},{"position":159,"residue":"TYR"},{"position":99,"residue":"TYR"},{"position":66,"residue":"ASN"},{"position":7,"residue":"TYR"},{"position":63,"residue":"GLU"}],"position":2,"residue":"SER"},"3":{"neighbours":[{"position":66,"residue":"ASN"},{"position":156,"residue":"LEU"},{"position":9,"residue":"TYR"},{"position":99,"residue":"TYR"},{"position":70,"residue":"SER"},{"position":159,"residue":"TYR"}],"position":3,"residue":"SER"},"4":{"neighbours":[{"position":159,"residue":"TYR"},{"position":66,"residue":"ASN"},{"position":163,"residue":"LEU"}],"position":4,"residue":"PRO"},"5":{"neighbours":[{"position":156,"residue":"LEU"},{"position":155,"residue":"GLN"},{"position":152,"residue":"VAL"},{"position":159,"residue":"TYR"}],"position":5,"residue":"VAL"},"6":{"neighbours":[{"position":73,"residue":"THR"},{"position":152,"residue":"VAL"}],"position":6,"residue":"THR"},"7":{"neighbours":[{"position":133,"residue":"TRP"},{"position":156,"residue":"LEU"},{"position":77,"residue":"ASN"},{"position":74,"residue":"TYR"},{"position":116,"residue":"SER"},{"position":73,"residue":"THR"},{"position":114,"residue":"ASP"},{"position":152,"residue":"VAL"},{"position":147,"residue":"TRP"}],"position":7,"residue":"LYS"},"8":{"neighbours":[{"position":146,"residue":"LYS"},{"position":77,"residue":"ASN"},{"position":73,"residue":"THR"},{"position":147,"residue":"TRP"},{"position":80,"residue":"ILE"},{"position":143,"residue":"THR"}],"position":8,"residue":"SER"},"9":{"neighbours":[{"position":143,"residue":"THR"},{"position":123,"residue":"TYR"},{"position":146,"residue":"LYS"},{"position":74,"residue":"TYR"},{"position":116,"residue":"SER"},{"position":80,"residue":"ILE"},{"position":84,"residue":"TYR"},{"position":77,"residue":"ASN"},{"position":95,"residue":"ILE"},{"position":142,"residue":"ILE"},{"position":147,"residue":"TRP"}],"position":9,"residue":"PHE"}},"peptide_sequence":"LSSPVTKSF","pockets":{"a":{"a159":{"number":159,"one_letter":"Y","three_letter":"TYR"},"a163":{"number":163,"one_letter":"L","three_letter":"LEU"},"a167":{"number":167,"one_letter":"W","three_letter":"TRP"},"a171":{"number":171,"one_letter":"Y","three_letter":"TYR"},"a5":{"number":5,"one_letter":"M","three_letter":"MET"},"a59":{"number":59,"one_letter":"Y","three_letter":"TYR"},"a63":{"number":63,"one_letter":"E","three_letter":"GLU"},"a66":{"number":66,"one_letter":"N","three_letter":"ASN"},"a7":{"number":7,"one_letter":"Y","three_letter":"TYR"}},"b":{"a24":{"number":24,"one_letter":"A","three_letter":"ALA"},"a34":{"number":34,"one_letter":"V","three_letter":"VAL"},"a45":{"number":45,"one_letter":"M","three_letter":"MET"},"a63":{"number":63,"one_letter":"E","three_letter":"GLU"},"a66":{"number":66,"one_letter":"N","three_letter":"ASN"},"a67":{"number":67,"one_letter":"M","three_letter":"MET"},"a7":{"number":7,"one_letter":"Y","three_letter":"TYR"},"a70":{"number":70,"one_letter":"S","three_letter":"SER"},"a9":{"number":9,"one_letter":"Y","three_letter":"TYR"},"a99":{"number":99,"one_letter":"Y","three_letter":"TYR"}},"c":{"a70":{"number":70,"one_letter":"S","three_letter":"SER"},"a73":{"number":73,"one_letter":"T","three_letter":"THR"},"a74":{"number":74,"one_letter":"Y","three_letter":"TYR"},"a9":{"number":9,"one_letter":"Y","three_letter":"TYR"},"a97":{"number":97,"one_letter":"V","three_letter":"VAL"}},"d":{"a114":{"number":114,"one_letter":"D","three_letter":"ASP"},"a155":{"number":155,"one_letter":"Q","three_letter":"GLN"},"a156":{"number":156,"one_letter":"L","three_letter":"LEU"},"a159":{"number":159,"one_letter":"Y","three_letter":"TYR"},"a160":{"number":160,"one_letter":"L","three_letter":"LEU"},"a99":{"number":99,"one_letter":"Y","three_letter":"TYR"}},"e":{"a114":{"number":114,"one_letter":"D","three_letter":"ASP"},"a147":{"number":147,"one_letter":"W","three_letter":"TRP"},"a152":{"number":152,"one_letter":"V","three_letter":"VAL"},"a156":{"number":156,"one_letter":"L","three_letter":"LEU"},"a97":{"number":97,"one_letter":"V","three_letter":"VAL"}},"f":{"a116":{"number":116,"one_letter":"S","three_letter":"SER"},"a123":{"number":123,"one_letter":"Y","three_letter":"TYR"},"a143":{"number":143,"one_letter":"T","three_letter":"THR"},"a146":{"number":146,"one_letter":"K","three_letter":"LYS"},"a147":{"number":147,"one_letter":"W","three_letter":"TRP"},"a77":{"number":77,"one_letter":"N","three_letter":"ASN"},"a80":{"number":80,"one_letter":"I","three_letter":"ILE"},"a81":{"number":81,"one_letter":"A","three_letter":"ALA"},"a84":{"number":84,"one_letter":"Y","three_letter":"TYR"},"a95":{"number":95,"one_letter":"I","three_letter":"ILE"}}},"publication":{"abstract":"Natural killer (NK) cells play a key role in immunity, but how HLA class I (HLA-I) and killer cell immunoglobulin-like receptor 3DL1 (KIR3DL1) polymorphism impacts disease outcome remains unclear. KIR3DL1 (*001/*005/*015) tetramers were screened for reactivity against a panel of HLA-I molecules. This revealed different and distinct hierarchies of specificity for each KIR3DL1 allotype, with KIR3DL1*005 recognizing the widest array of HLA-I ligands. These differences were further reflected in functional studies using NK clones expressing these specific KIR3DL1 allotypes. Unexpectedly, the Ile/Thr80 dimorphism in the Bw4-motif did not categorically define strong/weak KIR3DL1 recognition. Although the KIR3DL1*001, *005, and *015 polymorphisms are remote from the KIR3DL1-HLA-I interface, the structures of these three KIR3DL1-HLA-I complexes showed that the broader HLA-I specificity of KIR3DL1*005 correlated with an altered KIR3DL1*005 interdomain positioning and increased mobility within its ligand-binding site. Collectively, we provide a generic framework for understanding the impact of KIR3DL1 polymorphism on the recognition of HLA-I allomorphs.","bibjson":{"author":[{"initials":"PM","lastname":"Saunders","name":"Saunders PM"},{"initials":"P","lastname":"Pymm","name":"Pymm P"},{"initials":"G","lastname":"Pietra","name":"Pietra G"},{"initials":"VA","lastname":"Hughes","name":"Hughes VA"},{"initials":"C","lastname":"Hitchen","name":"Hitchen C"},{"initials":"GM","lastname":"O'Connor","name":"O'Connor GM"},{"initials":"F","lastname":"Loiacono","name":"Loiacono F"},{"initials":"J","lastname":"Widjaja","name":"Widjaja J"},{"initials":"DA","lastname":"Price","name":"Price DA"},{"initials":"M","lastname":"Falco","name":"Falco M"},{"initials":"MC","lastname":"Mingari","name":"Mingari MC"},{"initials":"L","lastname":"Moretta","name":"Moretta L"},{"initials":"DW","lastname":"McVicar","name":"McVicar DW"},{"initials":"J","lastname":"Rossjohn","name":"Rossjohn J"},{"initials":"AG","lastname":"Brooks","name":"Brooks AG"},{"initials":"JP","lastname":"Vivian","name":"Vivian JP"}],"identifier":[{"id":"10.1084/jem.20152023","type":"doi"},{"id":"27045007","type":"pubmed"}],"issue":[null],"journal":{"iso_abbreviation":"J. Exp. Med.","name":""},"pages":[null],"title":"Killer cell immunoglobulin-like receptor 3DL1 polymorphism defines distinct hierarchies of HLA class I recognition.","type":"article","url":"https://rupress.org/jem/article/213/5/791/42105/Killer-cell-immunoglobulinlike-receptor-3DL1","volume":[null],"year":[2016]},"in_pmc":"N","in_pmce":"Y","open_access":"Y"},"resolution":"2.50","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/5b39"},"rcsb":{"url":"https://www.rcsb.org/structure/5b39"}},"species":{"common_name":"Human","match_type":"histo:assign_species","scientific_name":"Homo sapiens","slug":"homo_sapiens"},"tcr":null,"title":"HLA-B*57:01 binding \"LSSPVTKSF\" with KIR-3 NK receptor at 2.50Å resolution","unique_chain_count":4}}