{"display":true,"structure":{"allele":{"alpha":{"identifier":"ipd-imgt:HLA35266","locus":"hla-a","match_type":"histo:exact","name":"HLA-A*02:01","slug":"hla_a_02_01","species_stem":"hla"},"beta":{}},"assemblies":{"1":{"assembly_name":"5d7i_1","chains":["A","B","G","H"],"downloaded_at":"2022-11-09T14:35:04.059208"}},"assembly_count":null,"assigned_chains":{"beta2m":{"chains":["B","D"],"match_type":"histo:assign_chains","score":0.99,"sequence":"MIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM"},"mr1":{"chains":["A","C"],"match_type":"histo:assign_chains","score":1.0,"sequence":"MRTHSLRYFRLGVSDPIHGVPEFISVGYVDSHPITTYDSVTRQKEPRAPWMAENLAPDHWERYTQLLRGWQQMFKVELKRLQRHYNHSGSHTYQRMIGCELLEDGSTTGFLQYAYDGQDFLIFNKDTLSWLAVDNVAHTIKQAWEANQHELLYQKNWLEEECIAWLKRFLEYGKDTLQRTEPPLVRVNRKETFPGVTALFCKAHGFYPPEIYMTWMKNGEEIVQEIDYGDILPSGDGTYQAWASIELDPQSSNLYSCHVEHSGVHMVLQVP"},"tcr_alpha":{"chains":["G"],"match_type":"stcrdab","sequence":"MGQNIDQPTEMTATEGAIVQINCTYQTSGFNGLFWYQQHAGEAPTFLSYNVLDGLEEKGRFSSFLSRSKGYSYLLLKELQMKDSASYLCAVMDSNYQLIWGAGTKLIIKPDIQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKCVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESS","subgroup":"TRAV1"},"tcr_beta":{"chains":["H"],"match_type":"stcrdab","sequence":"MIAGITQAPTSQILAAGRRMTLRCTQDMRHNAMYWYRQDLGLGLRLIHYSNTAGTTGKGEVPDGYSVSRANTDDFPLTLASAVPSQTSVYFCASSEAGGNTGELFFGEGSRLTVLEDLKNVFPPEVAVFEPSEAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVCTDPQPLKEQPALNDSRYALSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRAD","subgroup":"TRBV6"}},"chronology":{"deposition_date":"2015-08-14","deposition_year":2015,"release_date":"2016-01-27","release_year":2016,"revision_date":"2016-02-10","revision_year":2016},"class":"class_i","classical":false,"complex_type":"mr1_with_alpha_beta_tcr","components":["mr1","beta2m","tcr_alpha","tcr_beta"],"experimental_method":"X-ray diffraction","facets":{},"ligands":[],"locus":"hla-a","manually_edited":{},"missing_residues":[],"page_title":"5D7I | Non-classical MHC Class I molecule MR1 with Alpha/Beta T cell receptor","pdb_code":"5d7i","pdb_title":"Structure of human MR1-ac-6-fp in complex with human MAIT m33.64 TCR","peptide":{"actual_sequence":null,"epitope_info":{},"features":[],"full_sequence":null,"gap_info":{},"gapped_sequence":null,"length":{"numeric":null,"text":null},"unnatural_amino_acids":[]},"publication":{"abstract":"A characteristic of mucosal-associated invariant T (MAIT) cells is the expression of TRAV1-2(+) T cell receptors (TCRs) that are activated by riboflavin metabolite-based antigens (Ag) presented by the MHC-I related molecule, MR1. Whether the MR1-restricted T cell repertoire and associated Ag responsiveness extends beyond these cells remains unclear. Here, we describe MR1 autoreactivity and folate-derivative reactivity in a discrete subset of TRAV1-2(+) MAIT cells. This recognition was attributable to CDR3\u03b2 loop-mediated effects within a consensus TRAV1-2(+) TCR-MR1-Ag footprint. Furthermore, we have demonstrated differential folate- and riboflavin-derivative reactivity by a diverse population of \"atypical\" TRAV1-2(-) MR1-restricted T cells. We have shown that TRAV1-2(-) T cells are phenotypically heterogeneous and largely distinct from TRAV1-2(+) MAIT cells. A TRAV1-2(-) TCR docks more centrally on MR1, thereby adopting a markedly different molecular footprint to the TRAV1-2(+) TCR. Accordingly, diversity within the MR1-restricted T cell repertoire leads to differing MR1-restricted Ag specificity.","bibjson":{"author":[{"initials":"NA","lastname":"Gherardin","name":"Gherardin NA"},{"initials":"AN","lastname":"Keller","name":"Keller AN"},{"initials":"RE","lastname":"Woolley","name":"Woolley RE"},{"initials":"J","lastname":"Le Nours","name":"Le Nours J"},{"initials":"DS","lastname":"Ritchie","name":"Ritchie DS"},{"initials":"PJ","lastname":"Neeson","name":"Neeson PJ"},{"initials":"RW","lastname":"Birkinshaw","name":"Birkinshaw RW"},{"initials":"SBG","lastname":"Eckle","name":"Eckle SBG"},{"initials":"JN","lastname":"Waddington","name":"Waddington JN"},{"initials":"L","lastname":"Liu","name":"Liu L"},{"initials":"DP","lastname":"Fairlie","name":"Fairlie DP"},{"initials":"AP","lastname":"Uldrich","name":"Uldrich AP"},{"initials":"DG","lastname":"Pellicci","name":"Pellicci DG"},{"initials":"J","lastname":"McCluskey","name":"McCluskey J"},{"initials":"DI","lastname":"Godfrey","name":"Godfrey DI"},{"initials":"J","lastname":"Rossjohn","name":"Rossjohn J"}],"identifier":[{"id":"10.1016/j.immuni.2015.12.005","type":"doi"},{"id":"26795251","type":"pubmed"}],"issue":["1"],"journal":{"iso_abbreviation":"Immunity","name":""},"pages":["32-45"],"title":"Diversity of T Cells Restricted by the MHC Class I-Related Molecule MR1 Facilitates Differential Antigen Recognition.","type":"article","url":"https://www.sciencedirect.com/science/article/abs/pii/S1074761315005063","volume":["44"],"year":[2016]},"in_pmc":"N","in_pmce":"N","open_access":"N"},"resolution":"2.00","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/5d7i"},"rcsb":{"url":"https://www.rcsb.org/structure/5d7i"},"stcrdab":{"url":"http://opig.stats.ox.ac.uk/webapps/stcrdab/StrViewer?pdb=5d7i"}},"species":{"common_name":"Human","match_type":"histo:assign_species","scientific_name":"Homo sapiens","slug":"homo_sapiens"},"tcr":{"alpha":{"chains":["G"],"subgroup":"TRAV1"},"beta":{"chains":["H"],"subgroup":"TRBV6"},"pdb_code":"5d7i"},"title":"Non-classical MHC Class I molecule MR1 with Alpha/Beta T cell receptor at 2.00Å resolution","unique_chain_count":4}}