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coordinated recognition of virus-derived T cell epitopes and MHC molecules by T cells plays a pivotal role in cellular immunity-mediated virus clearance. It has been demonstrated that the conformation of MHC class I (MHC I) molecules can be adjusted by the presented peptide, which impacts T cell activation. However, it is still largely unknown whether the conformational shift of MHC I influences the protective effect of virus-specific T cells. In this study, utilizing the Middle East respiratory syndrome coronavirus-infected mouse model, we observed that through the unusual secondary anchor Ile5, a CD8<sup>+</sup> T cell epitope drove the conformational fit of Trp<sup>73</sup> on the \u03b11 helix of murine MHC I H-2K<sup>d</sup> In vitro renaturation and circular dichroism assays indicated that this shift of the structure did not influence the peptide/MHC I binding affinity. Nevertheless, the T cell recognition and the protective effect of the peptide diminished when we made an Ile to Ala mutation at position 5 of the original peptide. The molecular bases of the concordant recognition of T cell epitopes and host MHC-dependent protection were demonstrated through both crystal structure determination and tetramer staining using the peptide-MHC complex. Our results indicate a coordinated MHC I/peptide interaction mechanism and provide a beneficial reference for T cell-oriented vaccine development against emerging viruses such as Middle East respiratory syndrome coronavirus.","bibjson":{"author":[{"initials":"WJ","lastname":"Liu","name":"Liu WJ"},{"initials":"J","lastname":"Lan","name":"Lan J"},{"initials":"K","lastname":"Liu","name":"Liu K"},{"initials":"Y","lastname":"Deng","name":"Deng Y"},{"initials":"Y","lastname":"Yao","name":"Yao Y"},{"initials":"S","lastname":"Wu","name":"Wu S"},{"initials":"H","lastname":"Chen","name":"Chen H"},{"initials":"L","lastname":"Bao","name":"Bao L"},{"initials":"H","lastname":"Zhang","name":"Zhang H"},{"initials":"M","lastname":"Zhao","name":"Zhao M"},{"initials":"Q","lastname":"Wang","name":"Wang Q"},{"initials":"L","lastname":"Han","name":"Han L"},{"initials":"Y","lastname":"Chai","name":"Chai Y"},{"initials":"J","lastname":"Qi","name":"Qi J"},{"initials":"J","lastname":"Zhao","name":"Zhao J"},{"initials":"S","lastname":"Meng","name":"Meng S"},{"initials":"C","lastname":"Qin","name":"Qin C"},{"initials":"GF","lastname":"Gao","name":"Gao GF"},{"initials":"W","lastname":"Tan","name":"Tan W"}],"identifier":[{"id":"10.4049/jimmunol.1601542","type":"doi"},{"id":"27903740","type":"pubmed"}],"issue":["2"],"journal":{"iso_abbreviation":"J. 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