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changes of major histocompatibility complex (MHC) antigens have the potential to be recognized by T cells and may arise from polymorphic variation of the MHC molecule, the binding of modifying ligands, or both. Here, we investigated whether metal ions could affect allele-dependent structural variation of the two minimally distinct human leukocyte antigen (HLA)-B*27:05 and HLA-B*27:09 subtypes, which exhibit differential association with the rheumatic disease ankylosing spondylitis (AS). We employed NMR spectroscopy and X-ray crystallography coupled with ensemble refinement to study the AS-associated HLA-B*27:05 subtype and the AS-nonassociated HLA-B* 27:09 in complex with the self-peptide pVIPR (RRKWRRWHL). Both techniques revealed that pVIPR exhibits a higher degree of flexibility when complexed with HLA-B*27:05 than with HLA-B*27:09. Furthermore, we found that the binding of the metal ion Cu<sup>2+</sup> or Ni<sup>2+</sup>, but not Mn<sup>2+</sup>, Zn<sup>2+</sup>, or Hg<sup>2+</sup>, affects the structure of a pVIPR-bound HLA-B*27 molecule in a subtype-dependent manner. In HLA-B*27:05, the metals triggered conformational reorientations of pVIPR, but no such structural changes were observed in the HLA-B*27:09 subtype, with or without bound metal ion. These observations provide the first demonstration that not only major histocompatibility complex class II, but also class I, molecules can undergo metal ion-induced conformational alterations. Our findings suggest that metals may have a role in triggering rheumatic diseases such as AS and also have implications for the molecular basis of metal-induced hypersensitivities and allergies.","bibjson":{"author":[{"initials":"R","lastname":"Driller","name":"Driller R"},{"initials":"M","lastname":"Ballaschk","name":"Ballaschk M"},{"initials":"P","lastname":"Schmieder","name":"Schmieder P"},{"initials":"B","lastname":"Uchanska-Ziegler","name":"Uchanska-Ziegler B"},{"initials":"A","lastname":"Ziegler","name":"Ziegler A"},{"initials":"B","lastname":"Loll","name":"Loll B"}],"identifier":[{"id":"10.1074/jbc.RA119.008937","type":"doi"},{"id":"31296658","type":"pubmed"}],"issue":[null],"journal":{"iso_abbreviation":"J. Biol. Chem.","name":""},"pages":[null],"title":"Metal-triggered conformational reorientation of a self-peptide bound to a disease-associated HLA-B*27 subtype.","type":"article","url":"https://www.sciencedirect.com/science/article/abs/pii/S0021925820303835","volume":[null],"year":[2019]},"in_pmc":"N","in_pmce":"Y","open_access":"N"},"resolution":"1.91","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/5ib3"},"rcsb":{"url":"https://www.rcsb.org/structure/5ib3"}},"species":{"common_name":"Human","match_type":"histo:assign_species","scientific_name":"Homo sapiens","slug":"homo_sapiens"},"tcr":null,"title":"HLA-B*27:05 binding \"RRKWRRWHL\" at 1.91&#8491; resolution","unique_chain_count":3}}