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anti-viral T\u00a0cell response is drawn from the naive T\u00a0cell repertoire. During influenza infection, the CD8<sup>+</sup> T\u00a0cell response to an H-2D<sup>b</sup>-restricted nucleoprotein epitope (NP<sub>366</sub>) is characterized by preferential expansion of T\u00a0cells bearing TRBV13<sup>+</sup> T\u00a0cell receptors (TCRs) and avoidance of TRBV17<sup>+</sup> T\u00a0cells, despite the latter dominating the naive precursor repertoire. We found two TRBV17<sup>+</sup> TCRs that bound H-2D<sup>b</sup>-NP<sub>366</sub> with a 180\u00b0 reversed polarity compared to the canonical TCR-pMHC-I docking. The TRBV17 \u03b2-chain dominated the interaction and, whereas the complementarity determining region-3 (CDR3) loops exclusively mediated contacts with the MHC-I, peptide specificity was attributable to germline-encoded recognition. Nevertheless, the TRBV17<sup>+</sup> TCR exhibited moderate affinity toward H-2D<sup>b</sup>-NP<sub>366</sub> and was capable of signal transduction. Thus, the naive CD8<sup>+</sup> T\u00a0cell pool can comprise TCRs adopting reversed pMHC-I docking modes that limit their involvement in the immune response.","bibjson":{"author":[{"initials":"S","lastname":"Gras","name":"Gras S"},{"initials":"J","lastname":"Chadderton","name":"Chadderton J"},{"initials":"CM","lastname":"Del Campo","name":"Del Campo CM"},{"initials":"C","lastname":"Farenc","name":"Farenc C"},{"initials":"F","lastname":"Wiede","name":"Wiede F"},{"initials":"TM","lastname":"Josephs","name":"Josephs TM"},{"initials":"XYX","lastname":"Sng","name":"Sng XYX"},{"initials":"M","lastname":"Mirams","name":"Mirams M"},{"initials":"KA","lastname":"Watson","name":"Watson KA"},{"initials":"T","lastname":"Tiganis","name":"Tiganis T"},{"initials":"KM","lastname":"Quinn","name":"Quinn KM"},{"initials":"J","lastname":"Rossjohn","name":"Rossjohn J"},{"initials":"NL","lastname":"La Gruta","name":"La Gruta NL"}],"identifier":[{"id":"10.1016/j.immuni.2016.09.007","type":"doi"},{"id":"27717799","type":"pubmed"}],"issue":["4"],"journal":{"iso_abbreviation":"Immunity","name":""},"pages":["749-760"],"title":"Reversed T Cell Receptor Docking on a Major Histocompatibility Class I Complex Limits Involvement in the Immune Response.","type":"article","url":"https://www.sciencedirect.com/science/article/abs/pii/S107476131630351X","volume":["45"],"year":[2016]},"in_pmc":"N","in_pmce":"N","open_access":"N"},"resolution":"2.65","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/5swz"},"rcsb":{"url":"https://www.rcsb.org/structure/5swz"},"stcrdab":{"url":"http://opig.stats.ox.ac.uk/webapps/stcrdab/StrViewer?pdb=5swz"}},"species":{"common_name":"Mouse","match_type":"histo:assign_species","scientific_name":"Mus musculus","slug":"mus_musculus"},"tcr":{"alpha":{"chains":["D"],"subgroup":"TRAV14"},"beta":{"chains":["E"],"subgroup":"TRBV17"},"mhc_type":"class_i","pdb_code":"5swz"},"title":"H2-Db presenting \"ASNENMETM\" to Alpha/Beta T cell receptor at 2.65&#8491; resolution","unique_chain_count":5}}