{"display":true,"structure":{"allele":{"alpha":{"identifier":"uniprot:P01899","locus":"h2-d","match_type":"histo:exact","name":"H2-Db","slug":"h2_db","species_stem":"H2"},"beta":{}},"assemblies":{"1":{"assembly_name":"5tw2_1","chains":["A","B","C"],"downloaded_at":"2022-11-09T14:34:51.420216"}},"assembly_count":null,"assigned_chains":{"beta2m":{"chains":["B"],"match_type":"histo:assign_chains","score":1.0,"sequence":"IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVEMSDMSFSKDWSFYILAHTEFTPTETDTYACRVKHASMAEPKTVYWDRDM"},"cd1d":{"chains":["A"],"match_type":"histo:assign_chains","score":0.97,"sequence":"SEAQQKNYTFRCLQMSSFANRSWSRTDSVVWLGDLQTHRWSNDSATISFTKPWSQGKLSNQQWEKLQHMFQVYRVSFTRDIQELVKMMSPKEDYPIEIQLSAGCEMYPGNASESFLHVAFQGKYVVRFWGTSWQTVPGAPSWLDLPIKVLNADQGTSATVQMLLNDTCPLFVRGLLEAGKSDLEKQEKPVAWLSSVPSSAHGHRQLVCHVSGFYPKPVWVMWMRGDQEQQGTHRGDFLPNADETWYLQATLDVEAGEEAGLACRVKHSSLGGQDIILYWHHHHHH"}},"chronology":{"deposition_date":"2016-11-11","deposition_year":2016,"release_date":"2017-09-20","release_year":2017,"revision_date":"2020-07-29","revision_year":2020},"class":"class_i","classical":false,"complex_type":"cd1d","components":["cd1d","beta2m"],"experimental_method":"X-ray diffraction","facets":{},"ligands":[],"locus":"h2-d","manually_edited":{},"missing_residues":[],"page_title":"5TW2 | Non-classical MHC Class I molecule CD1d","pdb_code":"5tw2","pdb_title":"Structure of mouse CD1d with bound alpha-galactosylsphingamide jg168","peptide":{"actual_sequence":null,"epitope_info":{},"features":[],"full_sequence":null,"gap_info":{},"gapped_sequence":null,"length":{"numeric":null,"text":null},"unnatural_amino_acids":[]},"publication":{"abstract":"Invariant Natural Killer T-cells (iNKT-cells) are an attractive target for immune response modulation, as upon CD1d-mediated stimulation with KRN7000, a synthetic \u03b1-galactosylceramide, they produce a vast amount of cytokines. Here we present a synthesis that allows swift modification of the phytosphingosine side chain by amidation of an advanced methyl ester precursor. The resulting KRN7000 derivatives, termed \u03b1-galactosylsphingamides, were evaluated for their capacity to stimulate iNKT-cells. While introduction of the amide-motif in the phytosphingosine chain is tolerated for CD1d binding and TCR recognition, the studied \u03b1-galactosylsphingamides showed compromised antigenic properties.","bibjson":{"author":[{"initials":"J","lastname":"Guillaume","name":"Guillaume J"},{"initials":"J","lastname":"Wang","name":"Wang J"},{"initials":"J","lastname":"Janssens","name":"Janssens J"},{"initials":"SG","lastname":"Remesh","name":"Remesh SG"},{"initials":"MDP","lastname":"Risseeuw","name":"Risseeuw MDP"},{"initials":"T","lastname":"Decruy","name":"Decruy T"},{"initials":"M","lastname":"Froeyen","name":"Froeyen M"},{"initials":"D","lastname":"Elewaut","name":"Elewaut D"},{"initials":"DM","lastname":"Zajonc","name":"Zajonc DM"},{"initials":"SV","lastname":"Calenbergh","name":"Calenbergh SV"}],"identifier":[{"id":"10.1038/s41598-017-04461-7","type":"doi"},{"id":"28655912","type":"pubmed"}],"issue":["1"],"journal":{"iso_abbreviation":"Sci Rep","name":""},"pages":["4276"],"title":"Galactosylsphingamides: new \ufffd\ufffd-GalCer analogues to probe the F'-pocket of CD1d.","type":"article","url":"http://www.nature.com/articles/s41598-017-04461-7","volume":["7"],"year":[2017]},"in_pmc":"N","in_pmce":"Y","open_access":"Y"},"resolution":"1.75","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/5tw2"},"rcsb":{"url":"https://www.rcsb.org/structure/5tw2"}},"species":{"common_name":"Mouse","match_type":"histo:assign_species","scientific_name":"Mus musculus","slug":"mus_musculus"},"tcr":null,"title":"Non-classical MHC Class I molecule CD1d at 1.75Å resolution","unique_chain_count":2}}