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investigate CTL epitope applications in swine, SLA-1*1502-restricted peptide epitopes matching porcine reproductive and respiratory syndrome virus (PRRSV) strains were explored by crystallography, biochemistry, and the specific pathogen-free (SPF) swine experiments. First, nine predicted PRRSV peptides were tested by assembly of the peptide-SLA-1*1502 (pSLA-1*1502) complexes, and the crystal structure of the SLA-1*1502 complex with one peptide (NSP9-TMP9) was determined. The NSP9-TMP9 peptide conformation presented by pSLA-1*1502 is different from that of the peptides presented by the known pSLA-1*0401 and pSLA-3*hs0202 complexes. Two consecutive Pro residues make the turn between P3 and P4 of NSP9-TMP9 much sharper. The D pocket of pSLA-1*1502 is unique and is important for peptide binding. Next, the potential SLA-1*1502-restricted peptide epitopes matching four typical genetic PRRSV strains were identified based on the peptide-binding motif of SLA-1*1502 determined by structural analysis and alanine scanning of the NSP9-TMP9 peptide. The tetrameric complex of SLA-1*1502 and NSP9-TMP9 was constructed and examined. Finally, taking NSP9-TMP9 as an example, the CTL immunogenicity of the identified PRRSV peptide epitope was evaluated. The SPF swine expressing the SLA-1*1502 alleles were divided into three groups: modified live vaccine (MLV), MLV+NSP9-TMP9, and the blank control group. NSP9-TMP9 was determined as a PRRSV CTL epitope with strong immunogenicity by flow cytometry and IFN-\u03b3 expression. Our study developed an integrated approach to identify SLA-I-restricted CTL epitopes from various important viruses and is helpful in designing and applying effective peptide-based vaccines for swine.","bibjson":{"author":[{"initials":"X","lastname":"Pan","name":"Pan X"},{"initials":"N","lastname":"Zhang","name":"Zhang N"},{"initials":"X","lastname":"Wei","name":"Wei X"},{"initials":"Y","lastname":"Jiang","name":"Jiang Y"},{"initials":"R","lastname":"Chen","name":"Chen R"},{"initials":"Q","lastname":"Li","name":"Li Q"},{"initials":"R","lastname":"Liang","name":"Liang R"},{"initials":"L","lastname":"Zhang","name":"Zhang L"},{"initials":"L","lastname":"Ma","name":"Ma L"},{"initials":"C","lastname":"Xia","name":"Xia C"}],"identifier":[{"id":"10.3389/fimmu.2019.02995","type":"doi"},{"id":"31969884","type":"pubmed"}],"issue":[null],"journal":{"iso_abbreviation":"Front Immunol","name":""},"pages":["2995"],"title":"Illumination of PRRSV Cytotoxic T Lymphocyte Epitopes by the Three-Dimensional Structure and Peptidome of Swine Lymphocyte Antigen Class I (SLA-I).","type":"article","url":"https://www.frontiersin.org/article/10.3389/fimmu.2019.02995/full","volume":["10"],"year":[2019]},"in_pmc":"N","in_pmce":"Y","open_access":"Y"},"resolution":"2.20","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/5ylx"},"rcsb":{"url":"https://www.rcsb.org/structure/5ylx"}},"species":{"common_name":"Feral Pig","match_type":"histo:assign_species","scientific_name":"Sus scrofa","slug":"sus_scrofa"},"tcr":null,"title":"SLA-1*15:01 binding \"TMPPGFELY\" at 2.20Å resolution","unique_chain_count":3}}