{"display":true,"structure":{"allele":{"alpha":{"identifier":"ipd-mhc:NHP08956","locus":"mamu-b","match_type":"histo:fuzzy","name":"Mamu-B*098:08","slug":"mamu_b_098_08","species_stem":"Mamu"},"beta":{}},"assemblies":{"1":{"assembly_name":"6cug_1","chains":["A","B","D","E"],"downloaded_at":"2022-11-09T14:33:04.783330"}},"assembly_count":null,"assigned_chains":{"beta2m":{"chains":["B"],"match_type":"histo:assign_chains","score":1.0,"sequence":"IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM"},"cd1b":{"chains":["A"],"match_type":"histo:assign_chains","score":0.97,"sequence":"HAFQGPTSFHVIQTSSFTNSTWAQTQGSGWLDDLQIHGWDSDSGTAIFLKPWSKGNFSDKEVAELEEIFRVYIFGFAREVQDFAGDFQMKYPFEIQGIAGCELHSGGAIVSFLRGALGGLDFLSVKNASCVPSPEGGSRAQKFCALIIQYQGIMETVRILLYETCPRYLLGVLNAGKADLQRQVKPEAWLSSGPSPGPGRLQLVCHVSGFYPKPVWVMWMRGEQEQQGTQLGDILPNANWTWYLRATLDVADGEAAGLSCRVKHSSLEGQDIILYWRGSGLNDIFEAQKIEWHEHHHHHH"},"tcr_alpha":{"chains":["D"],"match_type":"stcrdab","sequence":"GNSVTQMEGPVTLSEEAFLTINCTYTATGYPSLFWYVQYPGEGLQLLLKATKADDKGSNKGFEATYRKETTSFHLEKGSVQVSDSAVYFCALTPSGGYQKVTFGTGTKLQVIPNIQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKCVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESS","subgroup":"TRAV9"},"tcr_beta":{"chains":["E"],"match_type":"stcrdab","sequence":"NAGVTQTPKFRVLKTGQSMTLLCAQDMNHEYMYWYRQDPGMGLRLIHYSVGEGTTAKGEVPDGYNVSRLKKQNFLLGLESAAPSQTSVYFCASSMPGLRSSYEQYFGPGTRLTVTEDLKNVFPPEVAVFEPSEAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVCTDPQPLKEQPALNDSRYALSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRAD","subgroup":"TRBV6"}},"chronology":{"deposition_date":"2018-03-26","deposition_year":2018,"release_date":"2019-01-16","release_year":2019,"revision_date":"2020-07-29","revision_year":2020},"class":"class_i","classical":false,"complex_type":"cd1b_with_nkt_alpha_beta_tcr","components":["cd1b","beta2m","tcr_alpha","tcr_beta"],"experimental_method":"X-ray diffraction","facets":{},"ligands":[],"locus":"mamu-b","manually_edited":{},"missing_residues":[],"page_title":"6CUG | Non-classical MHC Class I molecule CD1b with Natural Killer Alpha/Beta T cell receptor","pdb_code":"6cug","pdb_title":"Crystal structure of bc8b TCR-CD1b-pc complex","peptide":{"actual_sequence":null,"epitope_info":{},"features":[],"full_sequence":null,"gap_info":{},"gapped_sequence":null,"length":{"numeric":null,"text":null},"unnatural_amino_acids":[]},"publication":{"abstract":"CD1 proteins are expressed on dendritic cells, where they display lipid antigens to T-cell receptors (TCRs). Here we describe T-cell autoreactivity towards ubiquitous human membrane phospholipids presented by CD1b. These T-cells discriminate between two major types of lipids, sphingolipids and phospholipids, but were broadly cross-reactive towards diverse phospholipids including phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine. The crystal structure of a representative TCR bound to CD1b-phosphatidylcholine provides a molecular mechanism for this promiscuous recognition. We observe a lateral escape channel in the TCR, which shunted phospholipid head groups sideways along the CD1b-TCR interface, without contacting the TCR. Instead the TCR recognition site involved the neck region phosphate that is common to all major self-phospholipids but absent in sphingolipids. Whereas prior studies have focused on foreign lipids or rare self-lipids, we define a new molecular mechanism of promiscuous recognition of common self-phospholipids including those that are known targets in human autoimmune disease.","bibjson":{"author":[{"initials":"A","lastname":"Shahine","name":"Shahine A"},{"initials":"P","lastname":"Reinink","name":"Reinink P"},{"initials":"JF","lastname":"Reijneveld","name":"Reijneveld JF"},{"initials":"S","lastname":"Gras","name":"Gras S"},{"initials":"M","lastname":"Holzheimer","name":"Holzheimer M"},{"initials":"TY","lastname":"Cheng","name":"Cheng TY"},{"initials":"AJ","lastname":"Minnaard","name":"Minnaard AJ"},{"initials":"JD","lastname":"Altman","name":"Altman JD"},{"initials":"S","lastname":"Lenz","name":"Lenz S"},{"initials":"J","lastname":"Prandi","name":"Prandi J"},{"initials":"J","lastname":"Kubler-Kielb","name":"Kubler-Kielb J"},{"initials":"DB","lastname":"Moody","name":"Moody DB"},{"initials":"J","lastname":"Rossjohn","name":"Rossjohn J"},{"initials":"I","lastname":"Van Rhijn","name":"Van Rhijn I"}],"identifier":[{"id":"10.1038/s41467-018-07898-0","type":"doi"},{"id":"30610190","type":"pubmed"}],"issue":["1"],"journal":{"iso_abbreviation":"Nat Commun","name":""},"pages":["56"],"title":"A T-cell receptor escape channel allows broad T-cell response to CD1b and membrane phospholipids.","type":"article","url":"http://www.nature.com/articles/s41467-018-07898-0","volume":["10"],"year":[2019]},"in_pmc":"N","in_pmce":"Y","open_access":"Y"},"resolution":"2.40","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/6cug"},"rcsb":{"url":"https://www.rcsb.org/structure/6cug"},"stcrdab":{"url":"http://opig.stats.ox.ac.uk/webapps/stcrdab/StrViewer?pdb=6cug"}},"species":{"common_name":"Human","match_type":"histo:assign_species","scientific_name":"Homo sapiens","slug":"homo_sapiens"},"tcr":{"alpha":{"chains":["D"],"subgroup":"TRAV9"},"beta":{"chains":["E"],"subgroup":"TRBV6"},"pdb_code":"6cug"},"title":"Non-classical MHC Class I molecule CD1b with Natural Killer Alpha/Beta T cell receptor at 2.40Å resolution","unique_chain_count":4}}