{"display":true,"structure":{"allele":{"alpha":{"identifier":"ipd-imgt:HLA35266","locus":"hla-a","match_type":"histo:exact","name":"HLA-A*02:01","slug":"hla_a_02_01","species_stem":"hla"},"beta":{}},"assemblies":{"1":{"assembly_name":"6d7g_1","chains":["D","E","A_2"],"downloaded_at":"2022-11-09T14:32:22.493506"}},"assembly_count":null,"assigned_chains":{"class_i_alpha":{"chains":["A"],"match_type":"histo:assign_chains","score":0.78,"sequence":"ELAGIGILTVGGGGSGGGGSGGGGSIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDMGGGGSGGGGSGGGGSGGGGSGSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYWDGETRKVKAHSQTHRVDLGTLRGYYNQSEAGSHTVQRMYGCDVGSDWRFLRGYHQYAYDGKDYIALKEDLRSWTAADMAAQTTKHKWEAAHVAEQLRAYLEGTCVEWLRRYLENGKETLQRTDAPKTHMTHHAVSDHEATLRCWALSFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGQEQRYTCHVQHEGLPKPLTLRWEHHHHHHHH"},"tcr_delta":{"chains":["D"],"match_type":"stcrdab","sequence":"ADPAQKVTQAQSSVSMPVRKAVTLNCLYETSWWSYYIFWYKQLPSKEMIFLIRQGSDEQNAKSGRYSVNFKKAAKSVALTISALQLEDSAKYFCALGELGWDTDKLIFGKGTRVTVEPRSQPHTKPSVFVMKNGTNVACLVKEFYPKDIRINLVSSKKITEFDPAIVISPSGKYNAVKLGKYEDSNSVTCSVQHDNKTVHSTDFEVKTDSTDHVKPKETENTKQPSKSC","subgroup":"TRDV1"},"tcr_gamma":{"chains":["E"],"match_type":"stcrdab","sequence":"ADLSSNLEGRTKSVTRPTGSSAVITCDLPVENAVYTHWYLHQEGKAPQRLLYYDSYNSRVVLESGISREKYHTYASTGKSLKFILENLIERDSGVYYCATWASSDWIKTFAKGTRLIVTSPDKQLDADVSPKPTIFLPSIAETKLQKAGTYLCLLEKFFPDIIKIHWQEKKSNTILGSQEGNTMKTNDTYMKFSWLTVPEESLDKEHRCIVRHENNKNGIDQEIIFPPIKTDVTTVDPKYNYSKDANDVITMDPKDNC","subgroup":"TRGV8"}},"chronology":{"deposition_date":"2018-04-24","deposition_year":2018,"release_date":"2019-01-23","release_year":2019,"revision_date":"2020-07-29","revision_year":2020},"class":"class_i","classical":true,"complex_type":"single_chain_class_i_construct_with_gamma_delta_tcr","components":["class_i_alpha","tcr_delta","tcr_gamma"],"experimental_method":"X-ray 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recognition by T cells bearing \u03b1\u03b2 T cell receptors (TCRs) is restricted by major histocompatibility complex (MHC). However, how antigens are recognized by T cells bearing \u03b3\u03b4 TCRs remains unclear. Although \u03b3\u03b4 T cells can recognize nonclassical MHC, it is generally thought that recognition of antigens is not MHC restricted. Here, we took advantage of an in vitro system to generate antigen-specific human T cells and show that melanoma-associated antigens, MART-1 and gp100, can be recognized by \u03b3\u03b4 T cells in an MHC-restricted fashion. Cloning and transferring of MART-1-specific \u03b3\u03b4 TCRs restored the specific recognition of the initial antigen MHC/peptide reactivity and conferred antigen-specific functional responses. A crystal structure of a MART-1-specific \u03b3\u03b4 TCR, together with MHC/peptide, revealed distinctive but similar docking properties to those previously reported for \u03b1\u03b2 TCRs, recognizing MART-1 on HLA-A*0201. Our work shows that antigen-specific and MHC-restricted \u03b3\u03b4 T cells can be generated in vitro and that MART-1-specific \u03b3\u03b4 T cells can also be found and cloned from the na\u00efve repertoire. These findings reveal that classical MHC-restricted human \u03b3\u03b4 TCRs exist in the periphery and have the potential to be used in developing of new TCR-based immunotherapeutic approaches.","bibjson":{"author":[{"initials":"PM","lastname":"Benveniste","name":"Benveniste PM"},{"initials":"S","lastname":"Roy","name":"Roy S"},{"initials":"M","lastname":"Nakatsugawa","name":"Nakatsugawa M"},{"initials":"ELY","lastname":"Chen","name":"Chen ELY"},{"initials":"L","lastname":"Nguyen","name":"Nguyen L"},{"initials":"DG","lastname":"Millar","name":"Millar DG"},{"initials":"PS","lastname":"Ohashi","name":"Ohashi PS"},{"initials":"N","lastname":"Hirano","name":"Hirano N"},{"initials":"EJ","lastname":"Adams","name":"Adams EJ"},{"initials":"JC","lastname":"Z\ufffd\ufffd\ufffd\ufffdiga-Pfl\ufffd\ufffdcker","name":"Z\ufffd\ufffd\ufffd\ufffdiga-Pfl\ufffd\ufffdcker JC"}],"identifier":[{"id":"10.1126/sciimmunol.aav4036","type":"doi"},{"id":"30552102","type":"pubmed"}],"issue":["30"],"journal":{"iso_abbreviation":"Sci Immunol","name":""},"pages":[null],"title":"Generation and molecular recognition of melanoma-associated antigen-specific human \ufffd\ufffd\ufffd\ufffd T cells.","type":"article","url":"https://www.science.org/doi/10.1126/sciimmunol.aav4036","volume":["3"],"year":[2018]},"in_pmc":"N","in_pmce":"N","open_access":"N"},"resolution":"2.75","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/6d7g"},"rcsb":{"url":"https://www.rcsb.org/structure/6d7g"},"stcrdab":{"url":"http://opig.stats.ox.ac.uk/webapps/stcrdab/StrViewer?pdb=6d7g"}},"species":{"common_name":"Human","match_type":"histo:assign_species","scientific_name":"Homo sapiens","slug":"homo_sapiens"},"tcr":{"delta":{"chains":["D"],"subgroup":"TRDV1"},"gamma":{"chains":["E"],"subgroup":"TRGV8"},"pdb_code":"6d7g"},"title":"Single chain construct of HLA-A*02:01 presenting \"None\" to Gamma/Delta T cell receptor at 2.75Å resolution","unique_chain_count":3}}