{"display":true,"structure":{"allele":{"alpha":{"identifier":"ipd-imgt:HLA35266","locus":"hla-a","match_type":"histo:exact","name":"HLA-A*02:01","slug":"hla_a_02_01","species_stem":"hla"},"beta":{}},"assemblies":{"1":{"assembly_name":"6xqp_1","chains":["A","B","E","F"],"downloaded_at":"2022-11-09T14:30:43.859649"}},"assembly_count":null,"assigned_chains":{"beta2m":{"chains":["B","D"],"match_type":"histo:assign_chains","score":0.99,"sequence":"MIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM"},"mr1":{"chains":["A","C"],"match_type":"histo:assign_chains","score":1.0,"sequence":"MRTHSLRYFRLGVSDPIHGVPEFISVGYVDSHPITTYDSVTRQKEPRAPWMAENLAPDHWERYTQLLRGWQQMFKVELKRLQRHYNHSGSHTYQRMIGCELLEDGSTTGFLQYAYDGQDFLIFNKDTLSWLAVDNVAHTIKQAWEANQHELLYQKNWLEEECIAWLKRFLEYGKDTLQRTEPPLVRVNRKETFPGVTALFCKAHGFYPPEIYMTWMKNGEEIVQEIDYGDILPSGDGTYQAWASIELDPQSSNLYSCHVEHSGVHMVLQVP"},"tcr_alpha":{"chains":["E"],"match_type":"stcrdab","sequence":"MQKEVEQNSGPLSVPEGAIASLNCTYSDRGSQSFFWYRQYSGKSPELIMFIYSNGDKEDGRFTAQLNKASQYVSLLIRDSQPSDSATYLCAVRDAGNMLTFGGGTRLMVKPNIQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKCVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESS","subgroup":"TRAV12"},"tcr_beta":{"chains":["F"],"match_type":"stcrdab","sequence":"MSAVISQKPSRDICQRGTSLTIQCQVDSQVTMMFWYRQQPGQSLTLIATANQGSEATYESGFVIDKFPISRPNLTFSTLTVSNMSPEDSSIYLCSVGGDSLIGNQPQHFGDGTRLSILEDLKNVFPPEVAVFEPSEAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVCTDPQPLKEQPALNDSRYALSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRAD","subgroup":"TRBV29"}},"chronology":{"deposition_date":"2020-07-10","deposition_year":2020,"release_date":"2020-08-26","release_year":2020,"revision_date":"2020-10-28","revision_year":2020},"class":"class_i","classical":false,"complex_type":"mr1_with_alpha_beta_tcr","components":["mr1","beta2m","tcr_alpha","tcr_beta"],"experimental_method":"X-ray diffraction","facets":{},"ligands":[],"locus":"hla-a","manually_edited":{},"missing_residues":[],"page_title":"6XQP | Non-classical MHC Class I molecule MR1 with Alpha/Beta T cell receptor","pdb_code":"6xqp","pdb_title":"Structure of human d462-e4 TCR in complex with human MR1-5-op-ru","peptide":{"actual_sequence":null,"epitope_info":{},"features":[],"full_sequence":null,"gap_info":{},"gapped_sequence":null,"length":{"numeric":null,"text":null},"unnatural_amino_acids":[]},"publication":{"abstract":"MR1 presents vitamin B-related metabolites to mucosal associated invariant T (MAIT) cells, which are characterized, in part, by the TRAV1-2<sup>+</sup> \u03b1\u03b2 T cell receptor (TCR). In addition, a more diverse TRAV1-2<sup>-</sup> MR1-restricted T cell repertoire exists that can possess altered specificity for MR1 antigens. However, the molecular basis of how such TRAV1-2<sup>-</sup> TCRs interact with MR1-antigen complexes remains unclear. Here, we describe how a TRAV12-2<sup>+</sup> TCR (termed D462-E4) recognizes an MR1-antigen complex. We report the crystal structures of the unliganded D462-E4 TCR and its complex with MR1 presenting the riboflavin-based antigen 5-OP-RU. Here, the TRBV29-1 \u03b2-chain of the D462-E4 TCR binds over the F'-pocket of MR1, whereby the complementarity-determining region (CDR) 3\u03b2 loop surrounded and projected into the F'-pocket. Nevertheless, the CDR3\u03b2 loop anchored proximal to the MR1 A'-pocket and mediated direct contact with the 5-OP-RU antigen. The D462-E4 TCR footprint on MR1 contrasted that of the TRAV1-2<sup>+</sup> and TRAV36<sup>+</sup> TCRs' docking topologies on MR1. Accordingly, diverse MR1-restricted T cell repertoire reveals differential docking modalities on MR1, thus providing greater scope for differing antigen specificities.","bibjson":{"author":[{"initials":"W","lastname":"Awad","name":"Awad W"},{"initials":"EW","lastname":"Meermeier","name":"Meermeier EW"},{"initials":"ML","lastname":"Sandoval-Romero","name":"Sandoval-Romero ML"},{"initials":"J","lastname":"Le Nours","name":"Le Nours J"},{"initials":"AH","lastname":"Worley","name":"Worley AH"},{"initials":"MD","lastname":"Null","name":"Null MD"},{"initials":"L","lastname":"Liu","name":"Liu L"},{"initials":"J","lastname":"McCluskey","name":"McCluskey J"},{"initials":"DP","lastname":"Fairlie","name":"Fairlie DP"},{"initials":"DM","lastname":"Lewinsohn","name":"Lewinsohn DM"},{"initials":"J","lastname":"Rossjohn","name":"Rossjohn J"}],"identifier":[{"id":"10.1074/jbc.RA120.015292","type":"doi"},{"id":"32817339","type":"pubmed"}],"issue":[null],"journal":{"iso_abbreviation":"J. Biol. Chem.","name":""},"pages":[null],"title":"Atypical TRAV1-2- T cell receptor recognition of the antigen-presenting molecule MR1.","type":"article","url":"https://www.sciencedirect.com/science/article/abs/pii/S0021925817497857","volume":[null],"year":[2020]},"in_pmc":"N","in_pmce":"Y","open_access":"N"},"resolution":"2.90","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/6xqp"},"rcsb":{"url":"https://www.rcsb.org/structure/6xqp"},"stcrdab":{"url":"http://opig.stats.ox.ac.uk/webapps/stcrdab/StrViewer?pdb=6xqp"}},"species":{"common_name":"Human","match_type":"histo:assign_species","scientific_name":"Homo sapiens","slug":"homo_sapiens"},"tcr":{"alpha":{"chains":["E"],"subgroup":"TRAV12"},"beta":{"chains":["F"],"subgroup":"TRBV29"},"pdb_code":"6xqp"},"title":"Non-classical MHC Class I molecule MR1 with Alpha/Beta T cell receptor at 2.90&#8491; resolution","unique_chain_count":4}}