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T\u00a0cell receptor (TCR) expressed by T lymphocytes initiates protective immune responses to pathogens and tumors. To explore the structural basis of how TCR signaling is initiated when the receptor binds to peptide-loaded major histocompatibility complex (pMHC) molecules, we used cryogenic electron microscopy to determine the structure of a tumor-reactive TCR\u03b1\u03b2/CD3\u03b4\u03b3\u03b5<sub>2</sub>\u03b6<sub>2</sub> complex bound to a melanoma-specific human class I pMHC at 3.08\u00a0\u00c5 resolution. The antigen-bound complex comprises 11 subunits stabilized by multivalent interactions across three structural layers, with clustered membrane-proximal cystines stabilizing the CD3-\u03b5\u03b4 and CD3-\u03b5\u03b3 heterodimers. Extra density sandwiched between transmembrane helices reveals the involvement of sterol lipids in TCR assembly. The geometry of the pMHC/TCR complex suggests that efficient TCR scanning of pMHC requires accurate pre-positioning of T\u00a0cell and antigen-presenting cell membranes. Comparisons of the ligand-bound and unliganded receptors, along with molecular dynamics simulations, indicate that TCRs can be triggered in the absence of spontaneous structural rearrangements.","bibjson":{"author":[{"initials":"L","lastname":"Su\u0161ac","name":"Su\u0161ac L"},{"initials":"MT","lastname":"Vuong","name":"Vuong MT"},{"initials":"C","lastname":"Thomas","name":"Thomas C"},{"initials":"S","lastname":"von B\u00fclow","name":"von B\u00fclow S"},{"initials":"C","lastname":"O'Brien-Ball","name":"O'Brien-Ball C"},{"initials":"AM","lastname":"Santos","name":"Santos AM"},{"initials":"RA","lastname":"Fernandes","name":"Fernandes RA"},{"initials":"G","lastname":"Hummer","name":"Hummer G"},{"initials":"R","lastname":"Tamp\u00e9","name":"Tamp\u00e9 R"},{"initials":"SJ","lastname":"Davis","name":"Davis SJ"}],"identifier":[{"id":"10.1016/j.cell.2022.07.010","type":"doi"},{"id":"35985289","type":"pubmed"}],"issue":["17"],"journal":{"iso_abbreviation":"Cell","name":""},"pages":["3201-3213.e19"],"title":"Structure of a fully assembled tumor-specific T cell receptor ligated by pMHC.","type":"article","url":"https://www.sciencedirect.com/science/article/abs/pii/S0092867422009138","volume":["185"],"year":[2022]},"in_pmc":"N","in_pmce":"N","open_access":"N"},"resolution":"3.08","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/7phr"},"rcsb":{"url":"https://www.rcsb.org/structure/7phr"},"stcrdab":{"url":"http://opig.stats.ox.ac.uk/webapps/stcrdab/StrViewer?pdb=7phr"}},"species":{"common_name":"Human","match_type":"histo:override","scientific_name":"Homo sapiens","slug":"homo_sapiens"},"tcr":{"alpha":{"chains":["A"],"subgroup":"TRAV17"},"beta":{"chains":["B"],"subgroup":"TRBV19"},"mhc_type":"class_i","pdb_code":"7phr"},"title":"HLA-A*02:01 presenting \"YLEPGPVTV\" to Alpha/Beta T cell receptor at 3.08&#8491; resolution","unique_chain_count":5}}