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to antibody detection, severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2)-specific T-cell response evaluation is also pivotal among the coronavirus disease 2019 (COVID-19) convalescents and the vaccinated populations. Nucleocapsid (N) protein is one of the main structural proteins of SARS-CoV-2 and can trigger T-cell responses in humans.<h4>Methods</h4>An overlapping peptide pool covering the full length of the N protein was designed, peptides with positive T-cell activating potency in COVID-19 convalescents were screened, and CD8<sup>+</sup> T cell epitopes were further identified. The epitope was used to detect the SARS-CoV-2-specific CD8<sup>+</sup> T cell responses in COVID-19 convalescents based in intracellular cytokine staining and tetramer staining in flow cytometry.<h4>Results</h4>A human leukocyte antigen A (HLA-A)*1101-restricted CD8<sup>+</sup> T cell epitope, which could stimulate the production of IFN-\u03b3 via peripheral blood mononuclear cells (PBMCs) of the convalescents was defined, and the tetramer generated with this epitope could detect SARS-CoV-2-specific T cells in the PBMCs of the convalescents. The structural investigation eliminated that the epitope was a typical HLA-A*1101-restricted T-cell epitope which was conserved among all the sarbecoviruses.<h4>Discussion</h4>The newly identified SARS-CoV-2-derived T-cell epitope was helpful to detect the cellular immunity against different sarbecoviruses including SARS-CoV and SARS-CoV-2. This study provided an evaluation method and also a peptide candidate for the research and development of T-cell based vaccine for the virus.","bibjson":{"author":[{"initials":"J","lastname":"Zhang","name":"Zhang J"},{"initials":"D","lastname":"Lu","name":"Lu D"},{"initials":"M","lastname":"Li","name":"Li M"},{"initials":"M","lastname":"Liu","name":"Liu M"},{"initials":"S","lastname":"Yao","name":"Yao S"},{"initials":"J","lastname":"Zhan","name":"Zhan J"},{"initials":"WJ","lastname":"Liu","name":"Liu WJ"},{"initials":"GF","lastname":"Gao","name":"Gao GF"}],"identifier":[{"id":"10.46234/ccdcw2021.258","type":"doi"},{"id":"35186375","type":"pubmed"}],"issue":["5"],"journal":{"iso_abbreviation":"China CDC Wkly","name":""},"pages":["83-87"],"title":"A COVID-19 T-Cell Response Detection Method Based on a Newly Identified Human CD8<sub>+</sub> T Cell Epitope from SARS-CoV-2 - Hubei Province, China, 2021.","type":"article","url":"http://weekly.chinacdc.cn/en/article/doi/10.46234/ccdcw2021.258","volume":["4"],"year":[2022]},"in_pmc":"N","in_pmce":"Y","open_access":"Y"},"resolution":"1.50","same_as":{"pdbe":{"url":"https://www.ebi.ac.uk/pdbe/entry/pdb/7wkj"},"rcsb":{"url":"https://www.rcsb.org/structure/7wkj"}},"species":{"common_name":"Human","match_type":"histo:assign_species","scientific_name":"Homo sapiens","slug":"homo_sapiens"},"tcr":null,"title":"HLA-A*11:01 binding \"KTFPPTEPK\" at 1.50&#8491; resolution","unique_chain_count":3}}